Structure of PDB 5k85 Chain A

Receptor sequence

>5k85A (length=652) Species: 235443 (Cryptococcus neoformans var. grubii H99)

VHHVHPLPDSVPESEDLFAPPPRMQGKEGRPKPHIGPNYESYVKEWAKTV
GPNSDEWWAAKARETLDWYDDFKTVRAGGFEHGDVQWFPEGTLNAAYNCL
DRHYYKNPKKTAIIYEADEPSESREVSYEELMQETCRVANVLKSYGVKKG
DAVSIYLPMTWQAAAAFLACARIGAIHSAVFAGFSAESLRDRVNDCECKV
LITTDEGRRGGKTIATKQIVDAALQQCPLVENVLVLRRTGNKVPMTEGRD
KWWDEECAKMPAYCPCERMASEDPLFILYTSGKPKGVVHSTAGYLLGTAL
TLKYVFDAHPDDRFACMADIGWITGHSYIIYGPLANGITTAVFESTPVYP
TPSRYWDFVDKWKATQLYTAPTAIRLLRRMGEDHVKNHDLSSLRVLGSVG
EPINPEAWHWYNDFAGKNQCAIVDTYWMTETGSISIAPLPGAISTKPGSA
TFPFFGMDVDIIDPQTGQVLEGNDVEGVLVARRPWPSIARTVYRDHKRYL
ETYMKPYPGYFFFGDGAARDYDGYMWIKGRVDDVINVSGHRLSTAEVESA
LILHKGVAETAVVGCADDLTGQAVYAFVTMKPEFDLKATKEADLSKELAI
QVRKVIGPFAAPKKIYLVSDLPKTRSGKIMRRVLRKIVAGPQIVEEVKQK
VT

3D structure

• PDB: 5k85 CRYSTAL STRUCTURE OF ACETYL-COA SYNTHETASE IN COMPLEX WITH ADENOSINE-5'-PROPYLPHOSPHATE AND COENZYME A FROM CRYPTOCOCCUS NEOFORMANS H99
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T289 T441 E442 N548 R553 K640
• Catalytic site (residue number reindexed from 1): T280 T429 E430 N536 R541 K628
• Enzyme Commision number: 6.2.1.1: acetate--CoA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PRX	A	W334 I335 V411 G412 E413 P414 T437 Y438 W439 M440 T441 D527 R542	W322 I323 V399 G400 E401 P402 T425 Y426 W427 M428 T429 D515 R530

Gene Ontology

Molecular Function

• GO:0003987 acetate-CoA ligase activity
• GO:0005524 ATP binding
• GO:0016208 AMP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006085 acetyl-CoA biosynthetic process
• GO:0019427 acetyl-CoA biosynthetic process from acetate

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5k85, PDBe:5k85, PDBj:5k85
• PDBsum: 5k85
• UniProt: J9VFT1