Structure of PDB 5k3s Chain A

Receptor sequence

>5k3sA (length=583) Species: 3702 (Arabidopsis thaliana)

TFISRFAPDQPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSS
IRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADALLD
SVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRII
EEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPE
DSHLEQIVRLISESKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGL
GSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAF
ASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKL
DFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGV
GQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDI
DGDGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQWEDRFYKANRAH
TFLGDPAQEDEIFPNMLLFAAACGIPAARVTKKADLREAIQTMLDTPGPY
LLDVICPHQEHVLPMIPSGGTFNDVITEGDGRL

3D structure

• PDB: 5k3s Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families.
• Chain: A
• Resolution: 2.873 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y118 G120 G121 A122 S123 E144 T167 F206 Q207 E208 K256 M351 V378 V485 G511 M513 D538 N565 H567 L568 M570 V571 W574 H643
• Catalytic site (residue number reindexed from 1): Y33 G35 G36 A37 S38 E59 T82 F121 Q122 E123 K171 M266 V293 V400 G426 M428 D453 N480 H482 L483 M485 V486 W489 H558
• Enzyme Commision number: 2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	D538 N565 H567	D453 N480 H482
BS02	FAD	A	R246 G307 G308 G309 T331 L332 M333 L349 M351 G371 R373 D375 V378 D395 I396 D414 V415 M490 G508	R161 G222 G223 G224 T246 L247 M248 L264 M266 G286 R288 D290 V293 D310 I311 D329 V330 M405 G423
BS03	6QL	A	R377 M570 W574 G654	R292 M485 W489 G569
BS04	TP9	A	V485 G486 Q487 H488 G511 M513 D538 G539 S540 N565 H567 L568 G569 M570 V571	V400 G401 Q402 H403 G426 M428 D453 G454 S455 N480 H482 L483 G484 M485 V486

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0009082 branched-chain amino acid biosynthetic process

External links

• PDB: RCSB:5k3s, PDBe:5k3s, PDBj:5k3s
• PDBsum: 5k3s
• PubMed: 28137884
• UniProt: P17597|ILVB_ARATH Acetolactate synthase, chloroplastic (Gene Name=ALS)