Structure of PDB 5jpt Chain A

Receptor sequence
>5jptA (length=745) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
SKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVHHDAGEFKG
LQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEF
LKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVA
AMSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAME
DHDLSRYSCIILDEAHERTLATDILMGLLKQVVKRRPDLKIIIMSATLDA
EKFQRYFNDAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIHATEE
AGDILLFLTGEDEIEDAVRKISLEGDQLVREEGCGPLSVYPLYGSLPPHQ
QQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKVY
NPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELIEQ
SYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLA
CLDDEGNLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVP
NVFIRPTKDKKRADDAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKW
CRDHYLNYRSLSAADNIRSQLERLMNRYNLELNTTDYESPKYFDNIRKAL
ASGFFMQVAKKRSGGYITVKDNQDVLIHPSTVLGHDAEWVIYNEFVLTSK
NYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSLERIKEKVDR
3D structure
PDB5jpt Functional link between DEAH/RHA helicase Prp43 activation and ATP base binding.
ChainA
Resolution2.935 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CDP A G119 G121 K122 T123 T124 S155 V156 R159 F357 T384 D386 R430 G117 G119 K120 T121 T122 S153 V154 R157 F355 T382 D384 R428 PDBbind-CN: -logKd/Ki=4.60,Kd>25uM
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0003729 mRNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008186 ATP-dependent activity, acting on RNA
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000390 spliceosomal complex disassembly
GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0022613 ribonucleoprotein complex biogenesis
GO:0030490 maturation of SSU-rRNA
GO:0042273 ribosomal large subunit biogenesis
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005730 nucleolus
GO:0005739 mitochondrion
GO:0030686 90S preribosome
GO:0032040 small-subunit processome
GO:0071014 post-mRNA release spliceosomal complex
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5jpt, PDBe:5jpt, PDBj:5jpt
PDBsum5jpt
PubMed28180308
UniProtP53131|PRP43_YEAST Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (Gene Name=PRP43)

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