Structure of PDB 5jpi Chain A

Receptor sequence

>5jpiA (length=496) Species: 5807 (Cryptosporidium parvum)

AYKMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARIT
GSLHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVF
AWKNETIEDYWVCLNDAMTWRNPNDKDKICGPNLIVDDGGDATLILHEGV
KAEIEYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWR
GMLKDLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYG
CRQSLLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEI
DPICALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENA
YIANIGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKG
RLVNLGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKL
SKELDEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5jpi 2.15 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with D-Eritadenine and NAD.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H54 S77 S82 D138 E212 N237 K242 D246 N247 C251 H357 H411 S419 Q423
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	T212 T213 T214 N246 G275 G277 E278 V279 E298 I299 T331 N333 I354 G355 H356 N403	T213 T214 T215 N247 G276 G278 E279 V280 E299 I300 T332 N334 I355 G356 H357 N404
BS02	DEA	A	H53 T55 E57 E211 T212 K241 D245 L404 G409 H410 M415	H54 T56 E58 E212 T213 K242 D246 L405 G410 H411 M416
BS03	NAD	A	K489 Y493	K490 Y494

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5jpi, PDBe:5jpi, PDBj:5jpi
• PDBsum: 5jpi
• UniProt: Q5CPH1