Structure of PDB 5jiw Chain A

Receptor sequence

>5jiwA (length=500) Species: 271 (Thermus aquaticus)

MELPRAFGLLLHPTSLPGPYGVGVLGREARDFLRFLKEAGGRYWQVLPLG
PTGYGDSPYQSFSAFAGNPYLIDLRPLAERGYVRLEDPGFPQGRVDYGLL
YAWKWPALKEAFRGFKEKASPEEREAFAAFREREAWWLEDYALFMALKGA
HGGLPWNRWPLPLRKREEKALREAKSALAEEVAFHAFTQWLFFRQWGALK
AEAEALGIRIIGDMPIFVAEDSAEVWAHPEWFHLDEEGRPTVVAGVPPDY
FSETGQRWGNPLYRWDVLEREGFSFWIRRLEKALELFHLVRIAHFRGFEA
YWEIPASCPTAVEGRWVKAPGEKLFQKIQEVFGEVPVLAEDLGVITPEVE
ALRDRFGLPGMKVLQFAFDcGMENPFLPHNYPAHGRVVVYTGTHNNDTTL
GWYRTATPHEKAFMARYLADWGITFREEEEVPWALMHLGMKSVARLAVYP
VQDVLALGSEARMNYPGRPSGNWAWRLLPGELSPEHGARLRAMAEATERL

3D structure

• PDB: 5jiw Amylose recognition and ring-size determination of amylomaltase.
• Chain: A
• Resolution: 1.73 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A293 E340 N395
• Catalytic site (residue number reindexed from 1): A293 E340 N394
• Enzyme Commision number: 2.4.1.25: 4-alpha-glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	X370 N374	X370 N374
BS02	GLC	A	G343 D369 F376	G343 D369 F376
BS03	GLC	A	F251 G343	F251 G343
BS04	GLC	A	Q256 W258 H294 E340	Q256 W258 H294 E340
BS05	GLC	A	H394 N395	H394 N395
BS06	GLC	A	S57 Y59 Q60 N464	S57 Y59 Q60 N464
BS07	GLC	A	F62 G153	F62 G153
BS08	GLC	A	G55 D56	G55 D56

Gene Ontology

Molecular Function

• GO:0004134 4-alpha-glucanotransferase activity
• GO:0016757 glycosyltransferase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5jiw, PDBe:5jiw, PDBj:5jiw
• PDBsum: 5jiw
• PubMed: 28097217
• UniProt: O87172|MALQ_THETH 4-alpha-glucanotransferase (Gene Name=malQ)