Structure of PDB 5izm Chain A

Receptor sequence
>5izmA (length=460) Species: 9606 (Homo sapiens) [Search protein sequence]
GRRVNVNVGVLGHIDSGKTALARALSTTAITLDLGFSCFSVPLPARLRSS
PLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVI
GQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIP
VAAKPGGAPQGIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIGTVMTG
TILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQF
DPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHET
VMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSPRQQWALVEFEK
PVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLK
VYKLKHKDDYSVQLFVGLKVHLSTGELGIIDSFKIHIPGGLLSLTFKRYV
FDTHKRMVQS
3D structure
PDB5izm Crystal structures of the human elongation factor eEFSec suggest a non-canonical mechanism for selenocysteine incorporation.
ChainA
Resolution3.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D17 K20 T21 T48 H96
Catalytic site (residue number reindexed from 1) D15 K18 T19 T31 H63
Enzyme Commision number 3.6.5.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MN A T21 T48 T19 T31
BS02 GNP A D17 G19 K20 T21 A22 T48 N146 K147 D149 L150 A185 A186 K187 D15 G17 K18 T19 A20 T31 N113 K114 D116 L117 A152 A153 K154 PDBbind-CN: -logKd/Ki=5.92,Kd=1.2uM
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003746 translation elongation factor activity
GO:0003924 GTPase activity
GO:0005525 GTP binding
GO:0016787 hydrolase activity
GO:0035368 selenocysteine insertion sequence binding
GO:0043021 ribonucleoprotein complex binding
Biological Process
GO:0001514 selenocysteine incorporation
GO:0006412 translation
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:1990904 ribonucleoprotein complex

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Biological Process
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Cellular Component
External links
PDB RCSB:5izm, PDBe:5izm, PDBj:5izm
PDBsum5izm
PubMed27708257
UniProtP57772|SELB_HUMAN Selenocysteine-specific elongation factor (Gene Name=EEFSEC)

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