Structure of PDB 5iud Chain A

Receptor sequence

>5iudA (length=859) Species: 9606 (Homo sapiens)

EQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTL
YFLPREMKIDLNTGKETGTPISMKDVYEEFDEKIATKYKIMKFKSKPVEK
NYAFEIPDVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELF
LMNRKIKGPCWLEVKSPQLLNQPVSWCKVEAMALKPDLVNVIKDVSPPPL
VVMAFSMKTMQNAKNHQNEIIAMAALVHHSFALDKAAPKPPFQSHFCVVS
KPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGH
NIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGERNATCGRMICDV
EISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYLLE
HTWKDAKFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLL
HAFYENNYIVPDKQIKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQ
EFNICFTTVQRIPELPDPSLEMGILPREIRKLVERRKQVKQLMKQQDLNP
DLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMH
TKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLL
EIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCD
LAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEIN
KALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNL
TASQRAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIA
TWLGLDPTQ

3D structure

• PDB: 5iud Human DNA polymerase alpha in binary complex with a DNA:DNA template-primer.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R784 S785 A837 Y839 A840 G841 G842 G958 F962 K1052 K1054 R1081 D1148 S1151 S1189 R1222	R441 S442 A467 Y469 A470 G471 G472 G572 F576 K666 K668 R695 D762 S765 S803 R836
BS02	dna	A	R702 D1002 K1053 K1075 G1076 R1081 R1082 K1137 A1138 T1140 Y1146 H1154	R359 D616 K667 K689 G690 R695 R696 K751 A752 T754 Y760 H768

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:1902975 mitotic DNA replication initiation

External links

• PDB: RCSB:5iud, PDBe:5iud, PDBj:5iud
• PDBsum: 5iud
• PubMed: 27032819
• UniProt: P09884|DPOLA_HUMAN DNA polymerase alpha catalytic subunit (Gene Name=POLA1)