Structure of PDB 5ir4 Chain A

Receptor sequence

>5ir4A (length=658) Species: 287 (Pseudomonas aeruginosa)

DSIFFSPLKYLGAEQQRSIDASRSLLDNLIPPSLPQYDNLAGKLARRAVL
TSKKLVYVWTENFANVKGVPMARSVPLGELPNVDWLLKTAGVIVELIVNF
VASLPASAAAQFERIAAGLSGDLEAARQVHEALLEEAKNDPAAAGSLLLR
FTELQTRVIALLTRVGLLVDDILKSASNLVGLNRFRAVFGTLRLPEVADS
FRDDEAFAYWRVAGPNPLLIRRVDALPANFPLGEEQFRRVMGADDSLLEA
AASRRLYLLDYAELGKLAPSGAVDKLLTGTGFAYAPIALFALGKDRAGLL
PVAIQCGQDPATHPMFVRPAESESDLYWGWQMAKTVVQVAEENYHEMFVH
LAQTHLVSEAFCLATQRTLAPSHPLHVLLAPHFEGTLFINEGAARILLPS
AGFIDVMFAAPIQDTQATAGGNRLGFDFYRGMLPESLKARNVDDPAALPD
YPYRDDGLLVWNAIRQWAADYVAVYYASDGDVTADVELAAWVGEVIGSGK
VAGFRPITGRSQLVEVLTMVIFTASAQHAAVNFPQPSMMTYAPAICAMSA
APAPDSPSGKSEADWLKMMPPTLVALEKVNIYHLLGSVYHGRLGDYRQTG
FPYAPVFSDRRVTASGGPLERFQARLKEVEATIRTRNQARRKPYEYLLPS
RIPASTNI

3D structure

• PDB: 5ir4 Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
• Chain: A
• Resolution: 1.48 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H377 H382 H555 N559 I685
• Catalytic site (residue number reindexed from 1): H350 H355 H528 N532 I658
• Enzyme Commision number: 1.13.11.-
  1.13.11.33: arachidonate 15-lipoxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE2	A	H377 H382 H555 N559 I685	H350 H355 H528 N532 I658
BS02	ZPE	A	F120 L182 D190 H382 F415 I416 A420 L424	F100 L162 D170 H355 F388 I389 A393 L397

Gene Ontology

Molecular Function

• GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
• GO:0046872 metal ion binding

Biological Process

• GO:0034440 lipid oxidation

External links

• PDB: RCSB:5ir4, PDBe:5ir4, PDBj:5ir4
• PDBsum: 5ir4
• PubMed: 27500637
• UniProt: Q9I4G8|LOXA_PSEAE Lipoxygenase LoxA (Gene Name=loxA)