Structure of PDB 5ios Chain A

Receptor sequence
>5iosA (length=214) Species: 243274 (Thermotoga maritima MSB8) [Search protein sequence]
HMKIDILDKGFVELVDVMGNDLSAVRAARVSFLKDEERDRHLIEYLMKHG
HETPFEHIVFTFHVKAPIFVARQWFRHRIASYNELSGAYSKLSYEFYIPS
PERLEGYKTTIPPERVTEKISEIVDKAYRTYLELIESGVPREVARIVLPL
NLYTRFFWTVNARSLMNFLNLRADSHAQWEIQQYALAIARIFKEKCPWTF
EAFLKYAYKGDILK
3D structure
PDB5ios Deprotonations in the Reaction of Flavin-Dependent Thymidylate Synthase.
ChainA
Resolution1.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.148: thymidylate synthase (FAD).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A R78 H79 R80 I81 N169 L173 R174 R76 H77 R78 I79 N167 L171 R172
BS02 UMP A R74 Q75 R78 R174 R72 Q73 R76 R172 MOAD: Kd=14uM
BS03 UMP A E86 S88 G89 A90 R147 E84 S86 G87 A88 R145 MOAD: Kd=14uM
BS04 FAD A N85 E86 N83 E84
BS05 FAD A S30 T55 E58 I81 N163 R165 S31 T53 E56 I79 N161 R163
Gene Ontology
Molecular Function
GO:0004799 thymidylate synthase activity
GO:0008168 methyltransferase activity
GO:0050660 flavin adenine dinucleotide binding
GO:0050797 thymidylate synthase (FAD) activity
GO:0070402 NADPH binding
Biological Process
GO:0006231 dTMP biosynthetic process
GO:0006235 dTTP biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0032259 methylation

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Molecular Function
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Biological Process
External links
PDB RCSB:5ios, PDBe:5ios, PDBj:5ios
PDBsum5ios
PubMed27214228
UniProtQ9WYT0|THYX_THEMA Flavin-dependent thymidylate synthase (Gene Name=thyX)

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