Structure of PDB 5ims Chain A

Receptor sequence

>5imsA (length=541) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]

PDMDTSFVGLTGGQIFNEMMSRQNVDTVFGYPGGAILPVYDAIHNSDKFN
FVLPKHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVVTPMADAFADGI
PMVVFTGQVPTSAIGTDAFQEADVVGISRSCTKWNVMVKSVEELPLRINE
AFEIATSGRPGPVLVDLPKDVTAAILRNPIPTKTTLPSNRAQDEFVMQSI
NKAADLINLAKKPVLYVGAGILNHADGPRLLKELSDRAQIPVTTTLQGLG
SFDQEDPKSLDMLGMHGCATANLAVQNADLIIAVGARFDDRVTGNISKFA
PEARRAAAEGRGGIIHFEVSPKNINKVVQTQIAVEGDATTNLGKMMSKIF
PVKERSEWFAQINKWKKEYPYAYMEETPGSKIKPQTVIKKLSKVANDTGR
HVIVTTGVGQHQMWAAQHWTWRNPHTFITSGGLGTMGYGLPAAIGAQVAK
PESLVIDIDGDASFNMTLTELSSAVQAGTPVKILILNNEEHTHQLNPDFI
KLAEAMGLKGLRVKKQEELDAKLKEFVSTKGPVLLEVEVDK

3D structure

PDB

5ims A hidden redox cycle controls catalysis in acetohydroxyacid synthase

Chain

Resolution

1.984 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y113 G115 G116 A117 I118 E139 T162 F201 Q202 E203 K251 R318 M354 V381 V497 L522 G523 M525 D550 N577 E579 L608 G613 L614 K647
Catalytic site (residue number reindexed from 1)	Y31 G33 G34 A35 I36 E57 T80 F119 Q120 E121 K169 R229 M265 V292 V408 L433 G434 M436 D461 N488 E490 L502 G507 L508 K541
Enzyme Commision number	2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	FAD	A	R241 G307 A308 G309 N312 T334 L335 L352 G353 H355 G374 A375 R376 D378 R380 V381 E407 V408 N412 D426 A427	R159 G218 A219 G220 N223 T245 L246 L263 G264 H266 G285 A286 R287 D289 R291 V292 E318 V319 N323 D337 A338
BS02	MG	A	D550 N577	D461 N488
BS03	TPP	A	V497 G498 Q499 H500 G523 M525 G549 D550 A551 S552 M555 N577	V408 G409 Q410 H411 G434 M436 G460 D461 A462 S463 M466 N488
BS04	OXY	A	G116 T162 Q202	G34 T80 Q120
BS05	TPP	A	P114 E139	P32 E57

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0003984	acetolactate synthase activity
GO:0016740	transferase activity
GO:0030976	thiamine pyrophosphate binding
GO:0046872	metal ion binding
GO:0050660	flavin adenine dinucleotide binding

	Biological Process
GO:0008652	amino acid biosynthetic process
GO:0009082	branched-chain amino acid biosynthetic process
GO:0009097	isoleucine biosynthetic process
GO:0009099	L-valine biosynthetic process

	Cellular Component
GO:0005739	mitochondrion
GO:0005948	acetolactate synthase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:5ims, PDBe:5ims, PDBj:5ims
PDBsum	5ims
PubMed
UniProt	P07342\|ILVB_YEAST Acetolactate synthase catalytic subunit, mitochondrial (Gene Name=ILV2)

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