Structure of PDB 5ili Chain A

Receptor sequence
>5iliA (length=536) Species: 4097 (Nicotiana tabacum) [Search protein sequence]
IVRPVADFSPSLWGDQFLSFSIKNQVAEKYAKEIEALKEQTRNMLLATGM
KLADTLNLIDTIERLGISYHFEKEIDDILDQIYNQNSNCNDLCTSALQFR
LLRQHGFNISPEIFSKFQDENGKFKESLASDVLGLLNLYEASHVRTHADD
ILEDALAFSTIHLESAAPHLKSPLREQVTHALEQCLHKGVPRVETRFFIS
SIYDKEQSKNNVLLRFAKLDFNLLQMLHKQELAQVSRWWKDLDFVTTLPY
ARDRVVECYFWALGVYFEPQYSQARVMLVKTISMISIVDDTFDAYGTVKE
LEAYTDAIQRWDINEIDRLPDYMKISYKAILDLYKDYEKELSSAGRSHIV
CHAIERMKEVVRNYNVESTWFIEGYTPPVSEYLSNALATTTYYYLATTSY
LGMKSATEQDFEWLSKNPKILEASVIICRVIDDTATYEVEKSRGQIATGI
ECCMRDYGISTKEAMAKFQNMAETAWKDINEGLLRPTPVSTEFLTPILNL
ARIVEVTYIHNLDGYTHPEKVLKPHIINLLVDSIKI
3D structure
PDB5ili Small-molecule buffer components can directly affect terpene-synthase activity by interacting with the substrate-binding site of the enzyme
ChainA
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R264 W273 I294 D301 D305 T401 T402 T403 R441 D444 T448 E452 Y520 D525 Y527
Catalytic site (residue number reindexed from 1) R252 W261 I282 D289 D293 T389 T390 T391 R429 D432 T436 E440 Y508 D513 Y515
Enzyme Commision number 4.2.3.61: 5-epiaristolochene synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D444 T448 E452 D432 T436 E440
BS02 3FX A R441 D444 Y527 R429 D432 Y515
BS03 3CX A W273 T401 C440 R441 D444 Y527 W261 T389 C428 R429 D432 Y515
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0010333 terpene synthase activity
GO:0016829 lyase activity
GO:0016838 carbon-oxygen lyase activity, acting on phosphates
GO:0046872 metal ion binding
GO:0102698 5-epi-aristolochene synthase activity
Biological Process
GO:0006720 isoprenoid metabolic process
GO:0016102 diterpenoid biosynthetic process
GO:0016114 terpenoid biosynthetic process
GO:0051762 sesquiterpene biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Biological Process
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Cellular Component
External links
PDB RCSB:5ili, PDBe:5ili, PDBj:5ili
PDBsum5ili
PubMed
UniProtQ40577|5EAS_TOBAC 5-epi-aristolochene synthase (Gene Name=EAS3)

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