Structure of PDB 5iil Chain A

Receptor sequence

>5iilA (length=326) Species: 9606 (Homo sapiens)

TNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEA
CSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTA
QMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTV
QKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLD
SLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEF
ACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGR
VLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 5iil A fidelity mechanism in DNA polymerase lambda promotes error-free bypass of 8-oxo-dG.
• Chain: A
• Resolution: 1.96 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D178 D180 D241
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312	W25 R26 G29 Y30 P54 G55 G57 R59 M60 K63
BS02	dna	A	W342 G343 G345 K347 T348 D427 D429 Y505 T507 G508 A510 N513	W93 G94 G96 K98 T99 D178 D180 Y256 T258 G259 A261 N264
BS03	dna	A	W274 V462 S463 Q464 Q471 K472 R514 R517 K521 L527 S528 E529 H530 H541	W25 V213 S214 Q215 Q222 K223 R265 R268 K272 L278 S279 E280 H281 H292

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:5iil, PDBe:5iil, PDBj:5iil
• PDBsum: 5iil
• PubMed: 27481934
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)