Structure of PDB 5ifp Chain A

Receptor sequence

>5ifpA (length=968) Species: 425011 (Aspergillus niger CBS 513.88)

LLQKYVTWDDKSLFINGERIMIFSGEFHPFRLPVKELQLDIFQKVKALGF
NCVSFYVDWALVEGKPGEYRADGIFDLEPFFDAASEAGIYLLARPGPYIN
AESSGGGFPGWLQRVNGTLRSSDKAYLDATDNYVSHVAATIAKYQITNGG
PIILYQPENEYTSGCCGVEFPDPVYMQYVEDQARNAGVVIPLINNDASAS
GNNAPGTGKGAVDIYGHDSYPLGFDCANPTVWPSGDLPTNFRTLHLEQSP
TTPYAIVEFQGGSYDPWGGPGFAACSELLNNEFERVFYKNDFSFQIAIMN
LYMIFGGTNWGNLGYPNGYTSYDYGSAVTESRNITREKYSELKLLGNFAK
VSPGYLTASPGNLTTSGYADTTDLTVTPLLGNSTGSFFVVRHSDYSSEES
TSYKLRLPTSAGSVTIPQLGGTLTLNGRDSKIHVTDYNVSGTNIIYSTAE
VFTWKKFADGKVLVLYGGAGEHHELAISTKSNVTVIEGSESGISSKQTSS
SVVVGWDVSTTRRIIQVGDLKILLLDRNSAYNYWVPQLATDGTSPGFSTP
EKVASSIIVKAGYLVRTAYLKGSGLYLTADFNATTSVEVIGVPSTAKNLF
INGDKTSHTVDKNGIWSATVDYNAPDISLPSLKDLDWKYVDTLPEIQSSY
DDSLWPAADLKQTKNTLRSLTTPTSLYSSDYGFHTGYLLYRGHFTATGNE
STFAIDTQGGSAFGSSVWLNGTYLGSWTGLYANSDYNATYNLPQLQAGKT
YVITVVIDNMGLEENWTVGEDLMKTPRGILNFLLAGRPSSAISWKLTGNL
GGEDYEDKVRGPLNEGGLYAERQGFHQPEPPSQNWKSSSPLEGLSEAGIG
FYSASFDLDLPKGWDVPLFLNIGNSTTPSPYRVQVYVNGYQYAKYISNIG
PQTSFPVPEGILNYRGTNWLAVTLWALDSAGGKLESLELSYTTPVLTALG
EVESVDQPKYKKRKGAYH

3D structure

• PDB: 5ifp Structural features of Aspergillus niger beta-galactosidase define its activity against glycoside linkages.
• Chain: A
• Resolution: 1.71 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.23: beta-galactosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	A	N911 E978	N871 E938
BS02	MAN	A	E377 N568	E337 N528
BS03	MAN	A	E322 R325	E282 R285
BS04	MAN	A	W307 G308 Y771	W267 G268 Y731
BS05	MAN	A	Y771 A772	Y731 A732

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:5ifp, PDBe:5ifp, PDBj:5ifp
• PDBsum: 5ifp
• PubMed: 28391618
• UniProt: A2QAN3|BGALA_ASPNC Beta-galactosidase A (Gene Name=lacA)