Structure of PDB 5id6 Chain A

Receptor sequence

>5id6A (length=1209) Species: 1410628 (Lachnospiraceae bacterium ND2006)

AASKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKG
VKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEI
NLRKEIAKAFKGAAGYKSLFKKDIIETILPEAAKDEIALVNSFNGFTTAF
TGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHE
VQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESGEK
IKGLNEYINLYNAKTKQALPKFKPLYKQVEGYTSDEEVLEVFRNTLNKNS
EIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNLIRDKW
NAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEYADADLSVVE
KLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDS
VKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVT
QKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKY
AKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDI
QKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEK
YKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQIYNKDFSDK
SHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPAN
SPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINN
FNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKIC
ELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKS
NPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVN
LLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADYIKK
WKLYSYGNRIRIFAAAAWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQ
SDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYE
AQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEW
LEYAQTSVK

3D structure

• PDB: 5id6 The crystal structure of Cpf1 in complex with CRISPR RNA
• Chain: A
• Resolution: 2.382 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K15 T16 R18 F19 K20 K514 Y516 K518 N706 K707 S710 K712 H714 G715 T716 N718 L719 H720 R747 H759 I765 A766 N767 K768 N769 N772 K774 T777 L779 Y781 V783 Y784 K785 D786 R788 N861 N862 F863 Y872 L875 S905 Q906 H909 K953 K960	K16 T17 R19 F20 K21 K502 Y504 K506 N694 K695 S698 K700 H702 G703 T704 N706 L707 H708 R735 H747 I753 A754 N755 K756 N757 N760 K762 T765 L767 Y769 V771 Y772 K773 D774 R776 N849 N850 F851 Y860 L863 S893 Q894 H897 K941 K948	PDBbind-CN: Kd=2.86nM
BS02	MG	A	T716 P717	T704 P705

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding

External links

• PDB: RCSB:5id6, PDBe:5id6, PDBj:5id6
• PDBsum: 5id6
• PubMed: 27096363
• UniProt: A0A182DWE3