Structure of PDB 5hxw Chain A

Receptor sequence

>5hxwA (length=433) Species: 585 (Proteus vulgaris)

PRHGFVEGTEGALPKQADVVVVGAGILGIMTAINLVERGLSVVIVEKGNI
AGEQSSRFYGQAISYKMPDETFLLHHLGKHRWREMNAKVGIDTTYRTQGR
VEVPLDEEDLVNVRKWIDERSKNVGSDIPFKTRIIEGAELNQRLRGATTD
WKIAGFEEDSGSFDPEVATFVMAEYAKKMGVRIYTQCAARGLETQAGVIS
DVVTEKGAIKTSQVVVAGGVWSRLFMQNLNVDVPTLPAYQSQQLISGSPT
APGGNVALPGGIFFREQADGTYATSPRVIVAPVVKESFTYGYKYLPLLAL
PDFPVHISLNEQLINSFMQSTHWNLDEVSPFEQFRNMTALPDLPELNASL
EKLKAEFPAFKESKLIDQWSGAMAIAPDENPIISEVKEYPGLVINTATGW
GMTESPVSAELTADLLLGKKPVLDPKPFSLYRF

3D structure

• PDB: 5hxw Crystal structure of a membrane-bound l-amino acid deaminase from Proteus vulgaris
• Chain: A
• Resolution: 2.63 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): F96 Q99 A100 Q278 V322 F326
• Catalytic site (residue number reindexed from 1): F58 Q61 A62 Q240 V284 F288
• Enzyme Commision number: 1.4.3.2: L-amino-acid oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	V60 G61 G63 I64 L65 E84 K85 E91 Q92 S93 R95 F96 Y97 G98 Q99 A226 A227 G256 G257 W259 Q278 Q280 F369 G409 M411 T436 G437 W438 G439 M440 T441	V22 G23 G25 I26 L27 E46 K47 E53 Q54 S55 R57 F58 Y59 G60 Q61 A188 A189 G218 G219 W221 Q240 Q242 F331 G371 M373 T398 G399 W400 G401 M402 T403
BS02	16A	A	F355 M356 S358	F317 M318 S320
BS03	16A	A	N348 E349	N310 E311
BS04	16A	A	G329 Y332 M411 E417 G437 W438	G291 Y294 M373 E379 G399 W400

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5hxw, PDBe:5hxw, PDBj:5hxw
• PDBsum: 5hxw
• PubMed: 27422658
• UniProt: Q9LCB2