Structure of PDB 5htk Chain A

Receptor sequence
>5htkA (length=425) Species: 9606 (Homo sapiens) [Search protein sequence]
ASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRRE
AVKSYKSYDFFRHDNEEAMKIRKQCALVALEDVKAYLTEENGQIAVFDAT
NTTRERRDMILNFAEQNSFKVFFVESVCDDPDVIAANILEVKVSSPDYPE
RNRENVMEDFLKRIECYKVTYRPLDPDNYDKDLSFIKVINVGQRFLVNRV
QDYIQSKIVYYLMNIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQ
FAQALRKFLEEQEITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAG
VCEEMTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMEL
ERQGNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGC
KVETIKLNVEAVNTHRDKPTNNFPK
3D structure
PDB5htk Crystal structure of heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) and the inhibitory influence of citrate on substrate binding.
ChainA
Resolution2.01 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R256 H257 N263 R306 E326 H391
Catalytic site (residue number reindexed from 1) R226 H227 N233 R276 E296 H361
Enzyme Commision number 2.7.1.105: 6-phosphofructo-2-kinase.
3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FLC A R78 F91 R102 A129 T130 Y197 R48 F61 R72 A99 T100 Y167 MOAD: Kd=80uM
BS02 ATP A P47 A48 R49 G50 K51 T52 E170 S174 S175 P176 A427 Y428 P17 A18 R19 G20 K21 T22 E140 S144 S145 P146 A397 Y398
BS03 F6P A H257 I268 G269 E326 Y337 R351 K355 Y366 Q392 R396 H227 I238 G239 E296 Y307 R321 K325 Y336 Q362 R366
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003873 6-phosphofructo-2-kinase activity
GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016787 hydrolase activity
GO:0019900 kinase binding
GO:0019901 protein kinase binding
Biological Process
GO:0006000 fructose metabolic process
GO:0006003 fructose 2,6-bisphosphate metabolic process
GO:0006007 glucose catabolic process
GO:0006089 lactate metabolic process
GO:0006090 pyruvate metabolic process
GO:0006096 glycolytic process
GO:0009749 response to glucose
GO:0016310 phosphorylation
GO:0032024 positive regulation of insulin secretion
GO:0046835 carbohydrate phosphorylation
Cellular Component
GO:0005654 nucleoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5htk, PDBe:5htk, PDBj:5htk
PDBsum5htk
PubMed27802586
UniProtO60825|F262_HUMAN 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (Gene Name=PFKFB2)

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