Structure of PDB 5hrt Chain A

Receptor sequence

>5hrtA (length=786) Species: 10090 (Mus musculus)

TNTSGSCKGRCFELQDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDR
CGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPEC
PAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYP
TKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGG
QPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFY
SEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFV
GDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKA
IIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARK
PLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENI
ELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIM
YLQSDFDLGCTCDSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFL
MPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMS
YGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASE
RNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLD
FTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARV
RDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEI

3D structure

• PDB: 5hrt Structural basis for specific inhibition of Autotaxin by a DNA aptamer
• Chain: A
• Resolution: 1.997 Å

Enzymatic activity

• Enzyme Commision number: 3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R244 G245 R246 E247 H315 H452 A454 R455 P457 L458 S465 K467 F469 F470 D473	R188 G189 R190 E191 H259 H396 A398 R399 P401 L402 S409 K411 F413 F414 D417	PDBbind-CN: Kd=1.3nM
BS02	MAN	A	E223 S224 N436	E167 S168 N380
BS03	ZN	A	D171 T209 D358 H359	D115 T153 D302 H303
BS04	ZN	A	D311 H315 H474	D255 H259 H418
BS05	CA	A	D735 N737 N739 L741 D743	D663 N665 N667 L669 D671

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004528 phosphodiesterase I activity
• GO:0004551 dinucleotide phosphatase activity
• GO:0004622 lysophospholipase activity
• GO:0004630 phospholipase D activity
• GO:0005044 scavenger receptor activity
• GO:0005509 calcium ion binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0030247 polysaccharide binding
• GO:0046872 metal ion binding
• GO:0047391 alkylglycerophosphoethanolamine phosphodiesterase activity

Biological Process

• GO:0006644 phospholipid metabolic process
• GO:0006935 chemotaxis
• GO:0006955 immune response
• GO:0009395 phospholipid catabolic process
• GO:0010634 positive regulation of epithelial cell migration
• GO:0016042 lipid catabolic process
• GO:0016192 vesicle-mediated transport
• GO:0030149 sphingolipid catabolic process
• GO:0030334 regulation of cell migration
• GO:0034638 phosphatidylcholine catabolic process
• GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
• GO:2000394 positive regulation of lamellipodium morphogenesis

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0005886 plasma membrane

External links

• PDB: RCSB:5hrt, PDBe:5hrt, PDBj:5hrt
• PDBsum: 5hrt
• PubMed: 27043297
• UniProt: Q9R1E6|ENPP2_MOUSE Autotaxin (Gene Name=Enpp2)