Structure of PDB 5hr5 Chain A

Receptor sequence
>5hr5A (length=424) Species: 9913 (Bos taurus) [Search protein sequence]
KCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGV
YRRQAVKSYKSYDFFRHDNEEAMKIRKQCALVALKDVKAYLTEESGQIAV
FDATNTTRERRDLILNFAEENSFKVFFVESVCDDPDVIAANILEVKVSSP
DYPERNRENVMDDFLKRIECYKVTYQPLDPDSHDKDLSFIKVINVGQRFL
VNKVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSV
RGKQFAQALRKFLEEQEIADLKVWTSQLKRTIQTAESLGVTYEQWKILNE
IDAGVCEEMTYAEIQEQYPDEFALRDEEKYLYRYPGGESYQDLVQRLEPV
IMELERQGNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPV
AYGCKVETIKLNVEAVNTHRDKPT
3D structure
PDB5hr5 Crystal structure of heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) and the inhibitory influence of citrate on substrate binding.
ChainA
Resolution1.82 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R257 H258 N264 R307 E327 H392
Catalytic site (residue number reindexed from 1) R230 H231 N237 R280 E300 H365
Enzyme Commision number 2.7.1.105: 6-phosphofructo-2-kinase.
3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 F6P A H258 I269 G270 E327 Y338 R352 K356 Y367 Q393 R397 H231 I242 G243 E300 Y311 R325 K329 Y340 Q366 R370
BS02 FLC A G76 R79 R103 A130 T131 Y198 G49 R52 R76 A103 T104 Y171 MOAD: Ki=80uM
BS03 ADP A A49 R50 G51 K52 T53 Y54 S157 N168 E171 V172 K173 Y429 A22 R23 G24 K25 T26 Y27 S130 N141 E144 V145 K146 Y402
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003873 6-phosphofructo-2-kinase activity
GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016787 hydrolase activity
Biological Process
GO:0006000 fructose metabolic process
GO:0006003 fructose 2,6-bisphosphate metabolic process
GO:0006096 glycolytic process
GO:0016310 phosphorylation
GO:0046835 carbohydrate phosphorylation
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5hr5, PDBe:5hr5, PDBj:5hr5
PDBsum5hr5
PubMed27802586
UniProtP26285|F262_BOVIN 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (Gene Name=PFKFB2)

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