Structure of PDB 5hlk Chain A

Receptor sequence
>5hlkA (length=244) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB5hlk Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A N70 G109 T111 S112 K119 N120 T186 N69 G108 T110 S111 K118 N119 T185
BS02 dna A S183 G184 N185 T222 S223 R226 S182 G183 N184 T221 S222 R225
BS03 dna A T37 K92 T93 T94 Y95 T106 S183 T186 N188 T36 K91 T92 T93 Y94 T105 S182 T185 N187
BS04 dna A K38 Q68 K37 Q67
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:5hlk, PDBe:5hlk, PDBj:5hlk
PDBsum5hlk
PubMed27786446
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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