Structure of PDB 5hlb Chain A

Receptor sequence
>5hlbA (length=733) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
WLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPD
MTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKE
GQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFV
PRSGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGAS
TLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYL
GQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNP
KLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQ
LVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRK
LSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPAT
YLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNL
TPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQA
AYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGS
TVTITWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADM
GVDYDGNFVCSGGMRILPVWTSDPQSLCQQSEM
3D structure
PDB5hlb Escherichia coli Penicillin-Binding Protein 1B: Structural Insights into Inhibition.
ChainA
Resolution2.42 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.4.99.28: peptidoglycan glycosyltransferase.
3.4.16.4: serine-type D-Ala-D-Ala carboxypeptidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 M0E A G264 Q271 K274 N275 R286 Y315 E323 V354 K355 G356 S358 I359 G198 Q205 K208 N209 R220 Y249 E257 V288 K289 G290 S292 I293 MOAD: ic50=64.3nM
BS02 AZR A S507 S510 L611 T682 T699 G700 T701 T702 N703 S441 S444 L545 T616 T633 G634 T635 T636 N637 PDBbind-CN: -logKd/Ki=4.01,IC50=98.5uM
Gene Ontology
Molecular Function
GO:0008233 peptidase activity
GO:0008658 penicillin binding
GO:0008955 peptidoglycan glycosyltransferase activity
Biological Process
GO:0009252 peptidoglycan biosynthetic process
GO:0046677 response to antibiotic
Cellular Component
GO:0009274 peptidoglycan-based cell wall

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5hlb, PDBe:5hlb, PDBj:5hlb
PDBsum5hlb
PubMed27899450
UniProtP02919|PBPB_ECOLI Penicillin-binding protein 1B (Gene Name=mrcB)

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