Structure of PDB 5hje Chain A

Receptor sequence

>5hjeA (length=676) Species: 322104 (Scheffersomyces stipitis CBS 6054)

SSVDQKAISTIRLLAVDAVAAANSGHPGAPLGLAPAAHAVFKKMRFNPKD
TKWINRDRFVLSNGHACALLYSMLVLYGYDLTVEDLKKFRQLGSKTPGHP
ENTDVPGAEVTTGPLGQGICNGVGIALAQAQFAATYNKPDFPISDSYTYV
FLGDGCLMEGVSSEASSLAGHLQLGNLIAFWDDNKISIDGSTEVAFTEDV
IARYKSYGWHIVEVSDADTDITAIAAAIDEAKKVTNKPTLVRLTTTIGFG
SLAQGTHGVHGAPLKADDIKQLKTKWGFNPEESFAVPAEVTASYNEHVAE
NQKIQQQWNELFAAYKQKYPELGAELQRRLDGKLPENWDKALPVYTPADA
AVATRKLSEIVLSKIIPEVPEIIGGSADLTPSNLTKAKGTVDFQPAATGL
GDYSGRYIRYGVREHAMGAIMNGIAAFGANYKNYGGTFLNFVSYAAGAVR
LSALSEFPITWVATHDSIGLGEDGPTHQPIETLAHFRATPNISVWRPADG
NETSAAYKSAIESTHTPHILALTRQNLPQLEGSSIEKASKGGYTLVQQDK
ADIIIVATGSEVSLAVDALKVLEGQGIKAGVVSLPDQLTFDKQSEEYKLS
VLPDGVPILSVEVMSTFGWSKYSHQQFGLNRFGASGKAPEIFKLFEFTPE
GVAERAAKTVAFYKGKDVVSPLRSAF

3D structure

• PDB: 5hje Structural and biochemical interrogation on transketolase from Pichia stipitis for new functionality
• Chain: A
• Resolution: 1.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H27 I248 H261 E415 H478
• Catalytic site (residue number reindexed from 1): H26 I247 H260 E414 H477
• Enzyme Commision number: 2.2.1.1: transketolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPP	A	H66 G114 L116 D155 G156 N185 I187 I189 I248 H261	H65 G113 L115 D154 G155 N184 I186 I188 I247 H260
BS02	CA	A	D155 N185 I187	D154 N184 I186
BS03	I22	A	H27 I189 H261	H26 I188 H260

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004802 transketolase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006098 pentose-phosphate shunt

Cellular Component

• GO:0005634 nucleus
• GO:0005829 cytosol

External links

• PDB: RCSB:5hje, PDBe:5hje, PDBj:5hje
• PDBsum: 5hje
• PubMed: 27578891
• UniProt: P34736|TKT_PICST Transketolase (Gene Name=TKT)