Structure of PDB 5h9e Chain A

Receptor sequence

>5h9eA (length=481) Species: 83333 (Escherichia coli K-12)

NLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALL
VCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFM
QTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQ
ANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPT
ENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQ
ESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAP
SWTQISRVVVDKIIQNEGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQAS
ILERRHDVLMGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVS
VHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQ
SVAPYAHHPKLISTLALARATLYKHLRELKP

3D structure

• PDB: 5h9e Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.
• Chain: A
• Resolution: 3.21 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	F129 V130	F128 V129
BS02	dna	A	G159 G160 G161 F162 G168 T270 T301 T302 Q354 Y397 T398 E401 H416 L481 A484 T485 H489	G158 G159 G160 F161 G167 T265 T296 T297 Q348 Y383 T384 E387 H402 L467 A470 T471 H475
BS03	dna	A	N109 G123 A124 G159 G160 F162 K266 K268 Q354 A355 S356 K409 H472	N108 G122 A123 G158 G159 F161 K261 K263 Q348 A349 S350 K395 H458
BS04	ZN	A	C143 C250 C253	C142 C245 C248

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0051607 defense response to virus
• GO:0099048 CRISPR-cas system

Cellular Component

• GO:0032991 protein-containing complex

External links

• PDB: RCSB:5h9e, PDBe:5h9e, PDBj:5h9e
• PDBsum: 5h9e
• PubMed: 26863189
• UniProt: Q46901|CSE1_ECOLI CRISPR system Cascade subunit CasA (Gene Name=casA)