Structure of PDB 5h8y Chain A

Receptor sequence

>5h8yA (length=567) Species: 4577 (Zea mays) [Search protein sequence]

VKRSKVEIIKEKSNFLRYPLNEELVSEAPNINESAVQLIKFHGSYQQTDR
DVRGQKNYSFMLRTKNPCGKVPNQLYLAMDTLADEFGIGTLRLTTRQTFQ
LHGVLKKNLKTVLSTVIKNMGSTLGACGDLNRNVLAPAAPYVKKDILFAQ
QTAENIAALLTPQSGAYYDLWVDGEKIMSAEEPPEVTKARNDNSHGTNFP
DSPEPIYGTQYLPRKFKVAVTAAGDNSVDILTNDIGVVVVSDDAGEPIGF
NIYVGGGMGRTHRVETTFPRLADPLGYVPKEDILYAIKAIVVTQRENGRR
DDRKYSRMKYMIDRWGIDRFRAEVEKYYGKKFESFRPLPEWQFNSYLGWQ
EQGDGKLFYGVHVDNGRVGGQAKKTLREIIEKYNLDVSITPNQNLILCGI
DQAWREPITTALAQAGLLEPKDVDPLNLTAMACPALPLCPLAQTEAERGI
LPILKRIRAVFNKVGIKDSESVVVRITGCPNGCARPYMAELGFVGDGPKS
YQIWLGGTPNQSTLAESFMDKVKLDDIEKVLEPLFTYWNGTRQEGESFGS
FTNRTGFDKLKEVVNKW

3D structure

PDB

5h8y Structural and mutational studies of an electron transfer complex of maize sulfite reductase and ferredoxin.

Chain

Resolution

2.2 Å

3D
structure

Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Catalytic site (original residue number in PDB)	R124 R193 K276 K278 P452 C494 C500 C540 C544
Catalytic site (residue number reindexed from 1)	R63 R132 K215 K217 P391 C433 C439 C479 C483
Enzyme Commision number	1.8.7.1: assimilatory sulfite reductase (ferredoxin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	SF4	A	C494 A496 C500 A503 G539 C540 N542 C544	C433 A435 C439 A442 G478 C479 N481 C483
BS02	SRM	A	Y106 Q108 R124 R153 T155 T156 R157 T159 Q161 H163 R275 K276 K278 G318 M319 G320 R368 Q454 P495 C500 P501 N542 G543 C544 R546	Y45 Q47 R63 R92 T94 T95 R96 T98 Q100 H102 R214 K215 K217 G257 M258 G259 R307 Q393 P434 C439 P440 N481 G482 C483 R485
BS03	MG	A	V424 N453 N455	V363 N392 N394

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003690	double-stranded DNA binding
GO:0016002	sulfite reductase activity
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0046872	metal ion binding
GO:0050311	sulfite reductase (ferredoxin) activity
GO:0051536	iron-sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

	Biological Process
GO:0000103	sulfate assimilation
GO:0006790	sulfur compound metabolic process
GO:0009409	response to cold
GO:0019418	sulfide oxidation
GO:0045892	negative regulation of DNA-templated transcription
GO:1900160	plastid chromosome packaging

	Cellular Component
GO:0009337	sulfite reductase complex (NADPH)
GO:0009507	chloroplast
GO:0009570	chloroplast stroma
GO:0010319	stromule
GO:0042644	chloroplast nucleoid

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:5h8y, PDBe:5h8y, PDBj:5h8y
PDBsum	5h8y
PubMed	26920048
UniProt	O23813\|SIR_MAIZE Sulfite reductase [ferredoxin], chloroplastic (Gene Name=SIR)

[Back to BioLiP]