Structure of PDB 5guh Chain A

Receptor sequence

>5guhA (length=759) Species: 7091 (Bombyx mori) [Search protein sequence]

ISILRTRPEAVTSKKGTSGTPLDLLANYFTVETTPKWGLYQYHVDISPEE
DSTGVRKALMRVHSKTLGGYLFDGTVLYTVNRLHPDPMELYSDRKTDNER
MRILIKLTCEVSPGDYHYIQIFNIIIRKCFNLLKLQLMGRDYFDPEAKID
IPEFKLQIWPGYKTTINQYEDRLLLVTEIAHKVLRMDTVLQMLSEYAATK
KIFLEDVVGKIVMTDYNKRTYRVDDVANVSPKSTFKMRDENITYIEYYYK
KYNLRIQDPGQPLLISRSKPREIRAGLPELIYLVPELCRQTGLSDEMRAN
FKLMRSLDVHTKIGPDKRIEKLNNFNRRFTSTPEVVEELATWSLKLSKEL
VKIKGRQLPPENIIQANNVKYPAGDTTEGWTRDMRSKHLLAIAQLNSWVV
ITPERQRRDTESFIDLIIKTGGGVGFRMRSPDLVVIRHDGPIEYANMCEE
VIARKNPALILCVLARNYADRYEAIKKKCTVDRAVPTQVVCARNMSSKSA
MSIATKVAIQINCKLGGSPWTVDIPLPSLMVVGYDVCHDTRSKEKSFGAF
VATLDKQMTQYYSIVNAHLSSHMGFNIASAVKKFREKNGTYPARIFIYRD
GVGDGQIPYVHSHEVAEIKKKLAEIYAGVEIKLAFIIVSKRINTRIFVQR
GRSGENPRPGTVIDDVVTLPERYDFYLVSQNVREGTIAPTSYNVIEDTTG
LNPDRIQRLTYKLTHLYFNCSSQVRVPSVCQYAHKLAFLAANSLHNQPHY
SLNETLYFL

3D structure

PDB

5guh Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA

Chain

Resolution

2.4 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.26.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y350 Y355 F370 Y379 Y382 Y383 K386 Y387 E414 I416 Y417 N602 Y607 K611 Q623 V624 C626 N629 K641 V642 Q645 K649 Y857 N859 C860 Q871 L899	Y216 Y221 F235 Y244 Y247 Y248 K251 Y252 E279 I281 Y282 N467 Y472 K476 Q488 V489 C491 N494 K506 V507 Q510 K514 Y717 N719 C720 Q731 L759
BS02	MG	A	Q645 L899	Q510 L759

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004521	RNA endonuclease activity
GO:0005515	protein binding
GO:0034584	piRNA binding
GO:0046872	metal ion binding
GO:0061980	regulatory RNA binding

	Biological Process
GO:0007283	spermatogenesis
GO:0030154	cell differentiation
GO:0030237	female sex determination
GO:0031047	regulatory ncRNA-mediated gene silencing
GO:0034587	piRNA processing
GO:0051321	meiotic cell cycle
GO:0140965	secondary piRNA processing
GO:0140991	piRNA-mediated gene silencing by mRNA destabilization

	Cellular Component
GO:0005737	cytoplasm
GO:0043186	P granule
GO:1990923	PET complex

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:5guh, PDBe:5guh, PDBj:5guh
PDBsum	5guh
PubMed	27693359
UniProt	A8D8P8\|SIWI_BOMMO Piwi-like protein Siwi (Gene Name=Siwi)

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