Structure of PDB 5gr4 Chain A

Receptor sequence

>5gr4A (length=755) Species: 43989 (Crocosphaera subtropica ATCC 51142)

ISADQVNQIIYNLHHDPFEILGCHLLEEGKNTKKWVVRAYLPKAEAAWVI
RPTERKEDPMNSVHHPNFFECIIETPELNHYQLKVKEGEHEKVIYDPYAF
SSPYLTDEDIYLFSEGNHHRIYEKLGAHVGEINGVKGVYFAVWAPNARNV
SVIGDFNNWDGREHQMRKRNYTIWELFVPEIGSGTVYKYEIKNSEGHIYE
KSDPYGFYREVRPNTASIVVDIDNIYQWHDEEWLEKRRNSDPLKQPVSVY
EVHLGSWLHGSSAEKMPLLNGEADPVIVSEWNPGARFLSYYELAEKLIPY
VKDMGYTHIELLPIAEHPFDGSWGYQVTGFYSPTSRFGRPEDFMYFVDKC
HENGIGVILDWVPGHFPKDSHGLAYFDGTHLYEHADPRIGEHKEWGTLVF
NYGRHEVRNFLVANVLFWFDKYHVDGIRVDAVASMLYRNYLRKEGEWIAN
EYGGDEHIEAVSFIREVNTLLFEYFPGILSIAEESTEWEKVSRPVYDGGL
GFNLKWDMGWMHDMLDYFNIDPYFRQYHQNNVTFSMAYYYNENFMLALSH
DEIVHGKSNMLGKMPGDEWQKYANVRALFTYMYTHPGKKTMFMSMEFGQW
SEWNVWGDLEWHLLQYEPHQQLKQFFTDLNALYQQEPALYTHDFEYHGFE
WIDCNDNTHSVVSFLRRSDDPNDSLVVVCNFTPQPHSHYRIGVPEAGYYV
ELFNSDAKQYGGSNMGNLGGKWADEWSFHNKPYSLDLCLPPLAVLILKLD
PTKVP

3D structure

• PDB: 5gr4 Crystallization and crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.18: 1,4-alpha-glucan branching enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	G407 R408 H409	G403 R404 H405
BS02	GLC	A	R408 E410	R404 E406
BS03	GLC	A	N150 Y175	N146 Y171
BS04	GLC	A	W163 K192 E204	W159 K188 E200
BS05	GLC	A	W163 E214	W159 E210
BS06	GLC	A	F476 G505 F506 N507	F472 G501 F502 N503
BS07	GLC	A	P498 V499 Y500	P494 V495 Y496
BS08	GLC	A	D657 H733	D653 H729
BS09	GLC	A	Y693 R694	Y689 R690
BS10	GLC	A	S691 H692 G720 L722	S687 H688 G716 L718
BS11	GLC	A	E705 N718 G720 L722	E701 N714 G716 L718
BS12	GLC	A	E705 F707 N708 Y714	E701 F703 N704 Y710
BS13	GLC	A	D571 W573 Q574	D567 W569 Q570
BS14	GLC	A	P687 P689 S717	P683 P685 S713

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003844 1,4-alpha-glucan branching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016757 glycosyltransferase activity
• GO:0043169 cation binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005978 glycogen biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5gr4, PDBe:5gr4, PDBj:5gr4
• PDBsum: 5gr4
• UniProt: B1WPM8