Structure of PDB 5gqy Chain A

Receptor sequence

>5gqyA (length=755) Species: 43989 (Crocosphaera subtropica ATCC 51142)

ISADQVNQIIYNLHHDPFEILGCHLLEEGKNTKKWVVRAYLPKAEAAWVI
RPTERKEDPMNSVHHPNFFECIIETPELNHYQLKVKEGEHEKVIYDPYAF
SSPYLTDEDIYLFSEGNHHRIYEKLGAHVGEINGVKGVYFAVWAPNARNV
SVIGDFNNWDGREHQMRKRNYTIWELFVPEIGSGTVYKYEIKNSEGHIYE
KSDPYGFYREVRPNTASIVVDIDNIYQWHDEEWLEKRRNSDPLKQPVSVY
EVHLGSWLHGSSAEKMPLLNGEADPVIVSEWNPGARFLSYYELAEKLIPY
VKDMGYTHIELLPIAEHPFDGSWGYQVTGFYSPTSRFGRPEDFMYFVDKC
HENGIGVILDWVPGHFPKDSHGLAYFDGTHLYEHADPRIGEHKEWGTLVF
NYGRHEVRNFLVANVLFWFDKYHVDGIRVDAVASMLYRNYLRKEGEWIAN
EYGGDEHIEAVSFIREVNTLLFEYFPGILSIAEESTEWEKVSRPVYDGGL
GFNLKWDMGWMHDMLDYFNIDPYFRQYHQNNVTFSMLYYYNENFMLALSH
DEIVHGKSNMLGKMPGDEWQKYANVRALFTYMYTHPGKKTMFMSMEFGQW
SEWNVWGDLEWHLLQYEPHQQLKQFFTDLNALYQQEPALYTHDFEYHGFE
WIDCNDNTHSVVSFLRRSDDPNDSLVVVCNFTPQPHSHYRIGVPEAGYYV
ELFNSDAKQYGGSNMGNLGGKWADEWSFHNKPYSLDLCLPPLAVLILKLD
PTKVP

3D structure

• PDB: 5gqy Crystallization and crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.18: 1,4-alpha-glucan branching enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	G407 R408 H409	G403 R404 H405
BS02	GLC	A	R408 E410	R404 E406
BS03	GLC	A	N150 Y175	N146 Y171
BS04	GLC	A	W163 K192 E204	W159 K188 E200
BS05	GLC	A	W163 E214	W159 E210
BS06	GLC	A	F476 S496 P498 G505 F506 N507	F472 S492 P494 G501 F502 N503
BS07	GLC	A	F476 P498 V499 Y500	F472 P494 V495 Y496
BS08	GLC	A	R59 Y500	R55 Y496
BS09	GLC	A	D657 H733	D653 H729
BS10	GLC	A	Y693 R694	Y689 R690
BS11	GLC	A	S691 H692 G720 L722 C742	S687 H688 G716 L718 C738
BS12	GLC	A	E705 N718 G720 L722	E701 N714 G716 L718
BS13	GLC	A	E705 F707 N708 Y714	E701 F703 N704 Y710
BS14	GLC	A	D571 W573 Q574	D567 W569 Q570
BS15	GLC	A	P687 P689 S717 M719	P683 P685 S713 M715
BS16	GLC	A	T537 F538 L541	T533 F534 L537
BS17	GLC	A	M540 W655	M536 W651

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003844 1,4-alpha-glucan branching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016757 glycosyltransferase activity
• GO:0043169 cation binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005978 glycogen biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5gqy, PDBe:5gqy, PDBj:5gqy
• PDBsum: 5gqy
• UniProt: B1WPM8