Structure of PDB 5gke Chain A

Receptor sequence
>5gkeA (length=239) Species: 69014 (Thermococcus kodakarensis KOD1) [Search protein sequence]
SKDKVTVITSPSTEELVSLVNSALLEEAMLTIFARCKVHYDGRAKSELGS
GDRVIIVKPDGSFLIHQSKKREPVNWQPPGSRVRLELRENPVLVSIRRKP
RETLEVELEEVYMVSVFRAEDYEELALTGSEAEMAELIFENPEVIEPGFK
PLFREKAIGTGIVAVLGRDSDGNIVVLELKRRRAELHAVRQLKSYVEILR
EEYGDKVRGILVAPSLTSGAKRLLEKEGLEFRKLEPPKR
3D structure
PDB5gke Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease
ChainA
Resolution2.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K71 R72 E73 K100 L128 E132 G162 I163 K181 R182 R183 Q192 Y196 K70 R71 E72 K99 L127 E131 G161 I162 K180 R181 R182 Q191 Y195 PDBbind-CN: Kd=3.4nM
BS02 dna A Y41 R44 N76 W77 Q78 E103 R184 R191 T218 S219 G220 Y40 R43 N75 W76 Q77 E102 R183 R190 T217 S218 G219 PDBbind-CN: Kd=3.4nM
BS03 MG A E179 Q192 E178 Q191
Gene Ontology
Molecular Function
GO:0000014 single-stranded DNA endodeoxyribonuclease activity
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
Biological Process
GO:0006259 DNA metabolic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Cellular Component
External links
PDB RCSB:5gke, PDBe:5gke, PDBj:5gke
PDBsum5gke
PubMed27773688
UniProtQ5JER9|NUCS_THEKO Endonuclease NucS (Gene Name=nucS)

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