Structure of PDB 5fsj Chain A

Receptor sequence

>5fsjA (length=316) Species: 1427 (Bacillus thermoproteolyticus)

ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTL
PGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAI
RSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTD
YTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGD
SLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTH
YGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTS
QEVASVKQAFDAVGVK

3D structure

• PDB: 5fsj Gas-Sensitive Biological Crystals Processed in Pressurized Oxygen and Krypton Atmospheres: Deciphering Gas Channels in Proteins Using a Novel `Soak-and-Freeze' Methodology.
• Chain: A
• Resolution: 1.201 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H142 E143 H146 Y157 E166 D226 H231
• Catalytic site (residue number reindexed from 1): H142 E143 H146 Y157 E166 D226 H231
• Enzyme Commision number: 3.4.24.27: thermolysin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	VAL	A	N112 A113 E143 R203	N112 A113 E143 R203
BS02	LYS	A	N111 N112 F130 H231	N111 N112 F130 H231
BS03	CA	A	Y193 T194 I197 D200	Y193 T194 I197 D200
BS04	CA	A	E177 N183 D185 E190	E177 N183 D185 E190
BS05	CA	A	D59 Q61	D59 Q61
BS06	ZN	A	H142 H146 E166	H142 H146 E166
BS07	OXY	A	S92 Y93	S92 Y93
BS08	CA	A	D138 E177 D185 E187 E190	D138 E177 D185 E187 E190
BS09	CA	A	D57 D59 Q61	D57 D59 Q61

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:5fsj, PDBe:5fsj, PDBj:5fsj
• PDBsum: 5fsj
• UniProt: P00800|THER_BACTH Thermolysin (Gene Name=npr)