Structure of PDB 5few Chain A

Receptor sequence

>5fewA (length=346) Species: 2336 (Thermotoga maritima)

TGREILEKLERREFTREVLKEALSINDRGFNEALFKLADEIRRKYVGDEV
HIRAIIEFSNVCRKNCLYCGLRRDNKNLKRYRMTPEEIVERARLAVQFGA
KTIVLQSGEDPYYMPDVISDIVKEIKKMGVAVTLSLGEWPREYYEKWKEA
GADRYLLRHETANPVLHRKLRPDTSFENRLNCLLTLKELGYETGAGSMVG
LPGQTIDDLVDDLLFLKEHDFDMVGIGPFIPHPDTPLANEKKGDFTLTLK
MVALTRILLPDSNIPATTAMGTIVPGGREITLRCGANVIMPNWTPSPYRQ
LYQLYPGKISVFEKDTASIPSVMKMIELLGRKPGRDWGGRKRVFET

3D structure

• PDB: 5few Carbon-sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE.
• Chain: A
• Resolution: 1.17 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C63 C67 C70 V105 T134 G195 P266
• Catalytic site (residue number reindexed from 1): C62 C66 C69 V104 T133 G194 P265
• Enzyme Commision number: 1.8.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C63 K65 C67 C70	C62 K64 C66 C69
BS02	MET	A	Q107 S108 G138 Y303	Q106 S107 G137 Y302
BS03	5X8	A	Y69 R159 E161 M199 I231 T269 A270 Y306	Y68 R158 E160 M198 I230 T268 A269 Y305
BS04	SAH	A	Y69 S108 G138 R159 E161 M199 I231 L305 Y306	Y68 S107 G137 R158 E160 M198 I230 L304 Y305
BS05	41K	A	R159 T268 A270 M291 Y306	R158 T267 A269 M290 Y305

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0042364 water-soluble vitamin biosynthetic process
• GO:0044272 sulfur compound biosynthetic process

External links

• PDB: RCSB:5few, PDBe:5few, PDBj:5few
• PDBsum: 5few
• PubMed: 27102684
• UniProt: Q9X0Z6|HYDE_THEMA [FeFe] hydrogenase maturase subunit HydE (Gene Name=TM_1269)