Structure of PDB 5f9h Chain A

Receptor sequence

>5f9hA (length=646) Species: 9606 (Homo sapiens)

FKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQK
GKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVEN
NDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNY
LDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIA
TVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRIC
KDLREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKY
NDALIISEHARMKDALDYLKDFFSNVRAAFDEIEQDLTQRFEEKLQELES
VSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEG
NPKLSFLKPGILTGRDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKM
IQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEA
VFREKILHIQTHEKFIRDSQPKPVPDKENKKLLCRKCKALACYTADVRVI
EECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIH
VKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAE

3D structure

• PDB: 5f9h Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	I300 G326 T347 Q349 K379 Q380 H381 Q507 K508 E510 Q511 R546 K635 T636 R637 G663 T697 S698 V718 N720 S854 K888	I60 G86 T107 Q109 K139 Q140 H141 Q259 K260 E262 Q263 R298 K386 T387 R388 G414 T423 S424 V444 N446 S578 K612	PDBbind-CN: Kd=1.8nM
BS02	ZN	A	C810 C864 C869	C534 C588 C593
BS03	MG	A	P286 Q287 Q289	P46 Q47 Q49
BS04	MG	A	Q349 P382	Q109 P142
BS05	GTP	A	R664 H830 H847 F853 K858 K861 I875 K888	R415 H554 H571 F577 K582 K585 I599 K612

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f9h, PDBe:5f9h, PDBj:5f9h
• PDBsum: 5f9h
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)