Structure of PDB 5f8n Chain A

Receptor sequence

>5f8nA (length=462) Species: 39054 (Enterovirus A71)

GEIQWVKPNKETGRLNINGPTRTKLEPSVFHDVFEGNKEPAVLHSKDPRL
EVDFEQALFSKYVGNTLYEPDEYIKEAALHYANQLKQLDIDTSQMSMEEA
CYGTENLEAIDLHTSAGYPYSALGIKKRDILDSTTRDVSKMKFYMDKYGL
DLPYSTYVKDELRSIDKIKKGKSRLIEASSLNDSVYLRMTFGHLYETFHA
NPGTVTGSAVGCNPDTFWSKLPILLPGSLFAFDYSGYDASLSPVWFRALE
LVLREIGYSEEAVSLVEGINHTHHVYRNKTYCVLGGMPSGCSGTSIFNSM
INNIIIRALLIKTFKGIDLDELNMVAYGDDVLASYPFPIDCLELARTGKE
YGLTMTPADKSPCFNEVNWDNATFLKRGFLPDEQFPFLIHPTMPMKEIHE
SIRWTKDARNTQDHVRSLCLLAWHNGKQEYEKFVSAIRSVPVGKALAIPN
YENLRRNWLELF

3D structure

• PDB: 5f8n Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation
• Chain: A
• Resolution: 2.484 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P20 E108 T114 S115 S121 Y157 I176 A178 R188 H199 G211 C212 N213 G290 C291 S292 Y327	P20 E108 T114 S115 S121 Y157 I176 A178 R188 H199 G211 C212 N213 G290 C291 S292 Y327
BS02	rna	A	S133 K159 D238 S289 Y327 K376 N410 D413 H414 S417	S133 K159 D238 S289 Y327 K376 N410 D413 H414 S417
BS03	ZN	A	H271 H273 C282	H271 H273 C282
BS04	POP	A	R163 R174	R163 R174

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:5f8n, PDBe:5f8n, PDBj:5f8n
• PDBsum: 5f8n
• PubMed: 27339134
• UniProt: E5RPG2