Structure of PDB 5f8h Chain A

Receptor sequence

>5f8hA (length=462) Species: 39054 (Enterovirus A71)

GEIQWVKPNKETGRLNINGPTRTKLEPSVFHDVFEGNKEPAVLHSKDPRL
EVDFEQALFSKYVGNTLYEPDEYIKEAALHYANQLKQLDIDTSQMSMEEA
CYGTENLEAIDLHTSAGYPYSALGIKKRDILDSTTRDVSKMKFYMDKYGL
DLPYSTYVKDELRSIDKIKKGKSRLIEASSLNDSVYLRMTFGHLYETFHA
NPGTVTGSAVGCNPDTFWSKLPILLPGSLFAFDYSGYDASLSPVWFRALE
LVLREIGYSEEAVSLVEGINHTHHVYRNKTYCVLGGMPSGCSGTSIFNSM
INNIIIRALLIKTFKGIDLDELNMVAYGDDVLASYPFPIDCLELARTGKE
YGLTMTPADKSPCFNEVNWDNATFLKRGFLPDEQFPFLIHPTMPMKEIHE
SIRWTKDARNTQDHVRSLCLLAWHNGKQEYEKFVSAIRSVPVGKALAIPN
YENLRRNWLELF

3D structure

• PDB: 5f8h Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation
• Chain: A
• Resolution: 2.45 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P20 K24 E108 T114 S115 K127 Y157 K159 D160 I176 A178 R188 H199 G211 C212 N213 S289 G290 C291 S292 L420	P20 K24 E108 T114 S115 K127 Y157 K159 D160 I176 A178 R188 H199 G211 C212 N213 S289 G290 C291 S292 L420
BS02	rna	A	H113 Y327 G328 D329 D330 L375 K376 S401 N410 D413 H414 S417	H113 Y327 G328 D329 D330 L375 K376 S401 N410 D413 H414 S417
BS03	ZN	A	H271 H273 C282	H271 H273 C282

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:5f8h, PDBe:5f8h, PDBj:5f8h
• PDBsum: 5f8h
• PubMed: 27339134
• UniProt: E5RPG2