Structure of PDB 5f23 Chain A

Receptor sequence
>5f23A (length=263) Species: 208964 (Pseudomonas aeruginosa PAO1) [Search protein sequence]
HHHHHMQQIQRDIAQALQVQPPFQSEADVQAQIARRIAFIQQCLKDSGLK
TLVLGISGGVDSLTAGLLAQRAVEQLREQTGDQAYRFIAVRLPYQVQQDE
ADAQASLATIRADEEQTVNIGPSVKALAEQLEALEGLEPAKSDFVIGNIK
ARIRMVAQYAIAGARGGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLA
KHQVRALARALGAPENLVEKIHGVTYAEIDAFLHGQPLREEAARVIVDTY
HKTQHKRELPKAP
3D structure
PDB5f23 Crystal structure of NH(3)-dependent NAD(+) synthetase Pseudomonas aeruginosa in complex with NAD
ChainA
Resolution1.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D56 E172
Catalytic site (residue number reindexed from 1) D61 E177
Enzyme Commision number 6.3.1.5: NAD(+) synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAD A F139 N143 R147 F177 F178 T179 K180 H267 K268 F144 N148 R152 F182 F183 T184 K185 H255 K256
Gene Ontology
Molecular Function
GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
GO:0004359 glutaminase activity
GO:0005524 ATP binding
GO:0008795 NAD+ synthase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0009435 NAD biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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External links
PDB RCSB:5f23, PDBe:5f23, PDBj:5f23
PDBsum5f23
PubMed
UniProtQ9HUP3|NADE_PSEAE NH(3)-dependent NAD(+) synthetase (Gene Name=nadE)

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