Structure of PDB 5ey8 Chain A

Receptor sequence
>5ey8A (length=598) Species: 1772 (Mycolicibacterium smegmatis) [Search protein sequence]
GQIKFPDGSSIVAHVERWAKVRGDKLAYRFLDFSTERDGVPRDLTWAQFS
ARNRAVAARLQQVTQPGDRVAILCPQNLDYLVAFFGALYAGRIAVPLFDP
SEPGHVGRLHAVLDNCHPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVD
AVPDDVASTWVNPDEPDETTIAYLQYTSRIPTGVQITHLNLATNVVQVIE
ALEGEEGDRGLSWLPFFHDMGLITALLAPMIGHYFTFMTPAAFVRRPERW
IRELARKEGDTGGTISVAPNFAFDHAAARGVPKPGSPPLDLSNVKAVLNG
SEPISAATVRRFNEAFGPFGFPPKAIKPSYGLAEATLFVSTTPSAEEPKI
ITVDRDQLNSGRIVEVDADSPKAVAQASAGKVGIAEWAVIVDAESATELP
DGQVGEIWISGQNMGTGYWGKPEESVATFQNILKSRTNPSHAEGATDDAT
WVRTGDYGAFYDGDLYITGRVKDLVIIDGRNHYPQDLEYSAQEASKAIRT
GYVAAFSVPANQLPDEVFENAHSGIKRDPDDTSEQLVIVAERAPDIGPIT
DDIRAAIAVRHGVTVRDVLLTAAGAIPRTSSGKIGRRACRAAYLDGSL
3D structure
PDB5ey8 Insight into Structure-Function Relationships and Inhibition of the Fatty Acyl-AMP Ligase (FadD32) Orthologs from Mycobacteria.
ChainA
Resolution3.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N207 H231 A346
Catalytic site (residue number reindexed from 1) N194 H218 A333
Enzyme Commision number 6.2.1.20: long-chain-fatty-acid--[acyl-carrier-protein] ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 5SV A H231 D232 T237 S314 E315 P316 S342 Y343 G344 L350 D469 I480 H218 D219 T224 S301 E302 P303 S329 Y330 G331 L337 D456 I467 PDBbind-CN: -logKd/Ki=6.31,IC50=0.49uM
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008922 long-chain fatty acid [acyl-carrier-protein] ligase activity
GO:0016874 ligase activity
GO:0070566 adenylyltransferase activity
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006633 fatty acid biosynthetic process
GO:0008610 lipid biosynthetic process
Cellular Component
GO:0005886 plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5ey8, PDBe:5ey8, PDBj:5ey8
PDBsum5ey8
PubMed26900152
UniProtA0R618|FAA32_MYCS2 Long-chain-fatty-acid--AMP ligase FadD32 (Gene Name=fadD32)

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