Structure of PDB 5ewe Chain A

Receptor sequence

>5eweA (length=427) Species: 9606 (Homo sapiens)

GPHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGI
IAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASV
EVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYI
EGLPQVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIE
RETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKI
RSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGI
EHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKD
RNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKN
CNTSGIQTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 5ewe Mechanism of Ribonucleotide Incorporation by Human DNA Polymerase eta.
• Chain: A
• Resolution: 1.66 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W42 I47 S62 K86 R93 K311 R313 P316 K317 T318 I319 G320 C321 S322 K323 N324 P326 G327 R351	Q41 Y42 W45 I50 S65 K89 R96 K306 R308 P311 K312 T313 I314 G315 C316 S317 K318 N319 P321 G322 R346
BS02	dna	A	S113 E116 S257 G259 G260 K261 L262 R377 L381 R382	S116 E119 S252 G254 G255 K256 L257 R372 L376 R377
BS03	CTP	A	M14 D15 C16 F18 Y52 R55 R61 D115 K231	M17 D18 C19 F21 Y55 R58 R64 D118 K226
BS04	CA	A	D13 M14 D115	D16 M17 D118
BS05	CA	A	D13 D115 E116	D16 D118 E119

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:5ewe, PDBe:5ewe, PDBj:5ewe
• PDBsum: 5ewe
• PubMed: 26740629
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)