Structure of PDB 5emn Chain A

Receptor sequence

>5emnA (length=607) Species: 9606 (Homo sapiens)

SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEE
YDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGV
KFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF
ITWREQFWLAVCEHFGVEATGIRQYELVVHTDIDAAKVYMGEMGRLKSYE
NQKPPFDAKNPFLPAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHV
AVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTA
LTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWV
VEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVH
ICAVVVEYETKAGRINKGVATNWLRAKEPVGENGGRALVPMFVRKSQFRL
PFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSD
EDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLI
EGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGR
YSLDVWS

3D structure

• PDB: 5emn Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y459 S460 C632 D677 W679
• Catalytic site (residue number reindexed from 1): Y386 S387 C559 D604 W606
• Enzyme Commision number: 1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	A	S89 Q90 T91 T93 A94 T142 Y143 L176 G177 N178 Y181 F184	S20 Q21 T22 T24 A25 T73 Y74 L107 G108 N109 Y112 F115
BS02	FAD	A	R427 R457 Y458 Y459 S460 A476 Y481 G491 V492 A493 T494 W679	R354 R384 Y385 Y386 S387 A403 Y408 G418 V419 A420 T421 W606
BS03	NAP	A	R301 G537 T538 C569 S599 R600 K605 Y607 Q609 N637 M638 D641	R228 G464 T465 C496 S526 R527 K532 Y534 Q536 N564 M565 D568

Gene Ontology

Molecular Function

• GO:0003958 NADPH-hemoprotein reductase activity
• GO:0005515 protein binding
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

Biological Process

• GO:0006805 xenobiotic metabolic process
• GO:0009725 response to hormone
• GO:0022900 electron transport chain
• GO:0032770 positive regulation of monooxygenase activity
• GO:0090346 cellular organofluorine metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:5emn, PDBe:5emn, PDBj:5emn
• PDBsum: 5emn
• PubMed: 27496950
• UniProt: P16435|NCPR_HUMAN NADPH--cytochrome P450 reductase (Gene Name=POR)