Structure of PDB 5em4 Chain A

Receptor sequence
>5em4A (length=460) Species: 9986 (Oryctolagus cuniculus) [Search protein sequence]
KLPPGPSPLPVLGNLLQMDRKGLLRSFLRLREKYGDVFTVYLGSRPVVVL
CGTDAIREALVDQAEAFSGRGKIAVVDPIFQGYGVIFANGERWRALRRFS
LATMRDFGKRSVEERIQEEARCLVEELRKSKGALLDNTLLFHSITSNIIC
SIVFGKRFDYKDPVFLRLLDLFFQSFSLISSFSSQVFELFSGFLKYFPGT
HRQIYRNLQEINTWIGQSVEKHRATLDPSNPRDFIDVYLLRMEKDKSDPS
SEFHHQNLILTVLSLFFAGTETTSTTLRYGFLLMLKYPHVTERVQKEIEQ
VIGSHRPPALDDRAKMPYTDAVIHEIQRLGDLIPFGVPHTVTKDTQFRGY
VIPKNTEVFPVLSSALHDPRYFETPNTFNPGHFLDANGALKRNEGFMPFS
LGKRICLGEGIARTELFLFFTTILQNFSIASPVPPEDIDLTPSGVGNVPP
SYQIRFLARH
3D structure
PDB5em4 Coumarin Derivatives as Substrate Probes of Mammalian Cytochromes P450 2B4 and 2B6: Assessing the Importance of 7-Alkoxy Chain Length, Halogen Substitution, and Non-Active Site Mutations.
ChainA
Resolution3.02 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T302 F429 C436
Catalytic site (residue number reindexed from 1) T272 F399 C406
Enzyme Commision number 1.14.14.1: unspecific monooxygenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A R98 R125 G299 T303 I363 H369 F429 S430 R434 C436 L437 G438 A442 R70 R97 G269 T273 I333 H339 F399 S400 R404 C406 L407 G408 A412
BS02 CM5 A K186 F188 V194 F195 L198 F202 W244 K156 F158 V164 F165 L168 F172 W214
BS03 CM5 A L44 M46 D47 R48 F212 L16 M18 D19 R20 F182
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008392 arachidonate epoxygenase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
GO:0070330 aromatase activity
Biological Process
GO:0006805 xenobiotic metabolic process
GO:0019373 epoxygenase P450 pathway
Cellular Component
GO:0005737 cytoplasm
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5em4, PDBe:5em4, PDBj:5em4
PDBsum5em4
PubMed26982502
UniProtP00178|CP2B4_RABIT Cytochrome P450 2B4 (Gene Name=CYP2B4)

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