Structure of PDB 5ehf Chain A

Receptor sequence

>5ehfA (length=497) Species: 92699 (Antrodiella faginea)

AIGPVADLKIVNANIQPDGFTRPAVLAGGTFPGPLIKGNKGDNFQLNVID
ELENEDMLKSTSIHWHGFFQHGTNWADGPAFVNQCPITTGHSFLYNFHVP
DQAGTFWYHSHLSTQYCDGLRGPMVVYDPHDPLKQLYDVDDESTVMTLAD
WYHTLARQEPPGPVTPDSTLINGLGRAPGQTTPSELAVLTVKRGTRYRIR
LINISCEPNYHYSIDNHDLTVIEADGVSTQSLTVSSLTIFAGQRYSFILN
ANQPVGNYWIRAQPNDAADVTFNGGINSAILRYEGAPVAEPNTTAGPDNT
PLLEVNIRPFVFTPVPGQPHAGGADFVKNLLFSFNGTNFQVDNVSFVPPT
VPILLQILSGAHTAQDLMPAGSIIPLPKNAVIEFSMPGGVVGGGHPIHLH
GHNFWVIRSANSSVYNYNDPVIRDVVNIGTTGDNVTIRFETNNPGPWFLH
CHIDWHLDLGFAVVMAEDIPDAAAANPVPAAWNELCPLYDALTPGNQ

3D structure

• PDB: 5ehf Structure-function study of two new middle-redox potential laccases from basidiomycetes Antrodiella faginea and Steccherinum murashkinskyi.
• Chain: A
• Resolution: 1.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H64 H66 H109 H111 H395 H398 H400 H450 C451 H452 I453 H456 F461
• Catalytic site (residue number reindexed from 1): H64 H66 H109 H111 H395 H398 H400 H450 C451 H452 I453 H456 F461
• Enzyme Commision number: 1.10.3.2: laccase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CU	A	H111 H400 H450	H111 H400 H450
BS02	CU	A	H66 H109 H452	H66 H109 H452
BS03	CU	A	H64 H398 H400	H64 H398 H400
BS04	CU	A	H395 C451 H456	H395 C451 H456
BS05	ZN	A	H362 D366	H362 D366

Gene Ontology

Molecular Function

• GO:0005507 copper ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0052716 hydroquinone:oxygen oxidoreductase activity

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:5ehf, PDBe:5ehf, PDBj:5ehf
• PDBsum: 5ehf
• PubMed: 29890251
• UniProt: A0A0M3U1T9