Structure of PDB 5eau Chain A

Receptor sequence
>5eauA (length=523) Species: 4097 (Nicotiana tabacum) [Search protein sequence]
SPSLWGDQFLSFSIDNQVAEKYAKEIEALKEQTRNMLLATGMKLADTLNL
IDTIERLGISYHFEKEIDDILDQIYNQNSNCNDLCTSALQFRLLRQHGFN
ISPEIFSKFQDENGKFKESLASDVLGLLNLYEASHVRTHADDILEDALAF
STIHLESAAPHLKSPLREQVTHALEQCLHKGVPRVETRFFISSIYDKEQS
KNNVLLRFAKLDFNLLQMLHKQELAQVSRWWKDLDFVTTLPYARDRVVEC
YFWALGVYFEPQYSQARVMLVKTISMISIVDDTFDAYGTVKELEAYTDAI
QRWDINEIDRLPDYMKISYKAILDLYKDYEKELSSAGRSHIVCHAIERMK
EVVRNYNVESTWFIEGYTPPVSEYLSNALATTTYYYLATTSYLGMKSATE
QDFEWLSKNPKILEASVIICRVIDDTATYEVEKSRGQIATGIECCMRDYG
ISTKEAMAKFQNMAETAWKDINEGLLRPTPVSTEFLTPILNLARIVEVTY
IHNHPEKVLKPHIINLLVDSIKI
3D structure
PDB5eau Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.
ChainA
Resolution2.15 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R264 W273 I294 D301 D305 T401 T402 T403 R441 D444 T448 E452 Y520
Catalytic site (residue number reindexed from 1) R244 W253 I274 D281 D285 T381 T382 T383 R421 D424 T428 E432 Y500
Enzyme Commision number 4.2.3.61: 5-epiaristolochene synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D301 D305 D281 D285
BS02 MG A D444 T448 E452 D424 T428 E432
BS03 FFF A R264 W273 I294 D301 Y520 R244 W253 I274 D281 Y500
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0010333 terpene synthase activity
GO:0016829 lyase activity
GO:0016838 carbon-oxygen lyase activity, acting on phosphates
GO:0046872 metal ion binding
GO:0102698 5-epi-aristolochene synthase activity
Biological Process
GO:0006720 isoprenoid metabolic process
GO:0016102 diterpenoid biosynthetic process
GO:0016114 terpenoid biosynthetic process
GO:0051762 sesquiterpene biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5eau, PDBe:5eau, PDBj:5eau
PDBsum5eau
PubMed9295271
UniProtQ40577|5EAS_TOBAC 5-epi-aristolochene synthase (Gene Name=EAS3)

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