Structure of PDB 5eas Chain A

Receptor sequence
>5easA (length=514) Species: 4097 (Nicotiana tabacum) [Search protein sequence]
LWGDQFLSFSIDNQVAEKYAQEIEALKEQTRSMLLATGRKLADTLNLIDI
IERLGISYHFEKEIDEILDQIYNQNSNCNDLCTSALQFRLLRQHGFNISP
EIFSKFQDENGKFKESLASDVLGLLNLYEASHVRTHADDILEDALAFSTI
HLESAAPHLKSPLREQVTHALEQCLHKGVPRVETRFFISSIYDKEQSKNN
VLLRFAKLDFNLLQMLHKQELAQVSRWWKDLDFVTTLPYARDRVVECYFW
ALGVYFEPQYSQARVMLVKTISMISIVDDTFDAYGTVKELEAYTDAIQRW
DINEIDRLPDYMKISYKAILDLYKDYEKELSSAGRSHIVCHAIERMKEVV
RNYNVESTWFIEGYTPPVSEYLSNALATTTYYYLATTSYLGMKSATEQDF
EWLSKNPKILEASVIICRVIDDTATYEVEKSRGQIATGIECCMRDYGIST
KEAMAKFQNMAETAWKDINEGLLRPTPVSTEFLTPILNLARIVEVTYIVL
KPHIINLLVDSIKI
3D structure
PDB5eas Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.
ChainA
Resolution2.25 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) R264 W273 I294 D301 D305 T401 T402 T403 R441 D444 T448 E452 Y520
Catalytic site (residue number reindexed from 1) R241 W250 I271 D278 D282 T378 T379 T380 R418 D421 T425 E429 Y497
Enzyme Commision number 4.2.3.61: 5-epiaristolochene synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D444 T448 E452 D421 T425 E429
BS02 MG A D301 D305 D278 D282
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0010333 terpene synthase activity
GO:0016829 lyase activity
GO:0016838 carbon-oxygen lyase activity, acting on phosphates
GO:0046872 metal ion binding
GO:0102698 5-epi-aristolochene synthase activity
Biological Process
GO:0006720 isoprenoid metabolic process
GO:0016102 diterpenoid biosynthetic process
GO:0016114 terpenoid biosynthetic process
GO:0051762 sesquiterpene biosynthetic process
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:5eas, PDBe:5eas, PDBj:5eas
PDBsum5eas
PubMed9295271
UniProtQ40577|5EAS_TOBAC 5-epi-aristolochene synthase (Gene Name=EAS3)

[Back to BioLiP]