Structure of PDB 5ean Chain A

Receptor sequence

>5eanA (length=1051) Species: 10090 (Mus musculus)

GGHMEPLDELDLLLLEAVPRVELLRKKADALFPETVLSRGVDNRYLVLAV
ETSQNERGAEEKRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGD
CTSEPWIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRGSDPA
TRQMLIGTILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLS
QDEILCEVEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGRSSPCNIE
VVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKE
SNSIEHRSQVVLYTLLSQERREDPEAGWLLYLKTGQMYPVPANHLDKREL
LKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCAL
YSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLESQS
KDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKN
GPMPATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTL
PATTVFRLDREERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFI
AYLSSVLPHDAKDTVANILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGK
TTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHK
VHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRK
TFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVVNREARALG
MSESLFKRLERNESAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVA
NAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPE
QVENGGVSNVTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLL
ARSSVGMVEVNTVDKYQGRDKSLILVSFVRSNEDGTLGELLKDWRRLNVA
LTRAKHKLILLGSVSSLKRFPPLGTLFDHLNAEQLILDLPSREHESLSHI
L

3D structure

• PDB: 5ean Dna2 nuclease-helicase structure, mechanism and regulation by Rpa.
• Chain: A
• Resolution: 2.36 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S127 T129 M157 T161 H164 K274 G275 K276 D278 K301 T302 Y318 S398 R518 R568 Y678 T679 H680 T743 M745 L795 G910 R945 T967 D969 K970 G993 E994 L995 R1001	S124 T126 M154 T158 H161 K269 G270 K271 D273 K296 T297 Y313 S393 R513 R563 Y673 T674 H675 T738 M740 L790 G905 R940 T962 D964 K965 G988 E989 L990 R996
BS02	SF4	A	C137 R140 I388 C394 C397 C403 M423	C134 R137 I383 C389 C392 C398 M418
BS03	CA	A	H164 D278 E299 L300	H161 D273 E294 L295
BS04	ADP	A	G626 L627 Q631 G654 T656 T657 K689 Y828	G621 L622 Q626 G649 T651 T652 K684 Y823

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0004518 nuclease activity
• GO:0004519 endonuclease activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
• GO:0017108 5'-flap endonuclease activity
• GO:0017116 single-stranded DNA helicase activity
• GO:0043139 5'-3' DNA helicase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0000076 DNA replication checkpoint signaling
• GO:0000723 telomere maintenance
• GO:0000729 DNA double-strand break processing
• GO:0006259 DNA metabolic process
• GO:0006260 DNA replication
• GO:0006264 mitochondrial DNA replication
• GO:0006281 DNA repair
• GO:0006284 base-excision repair
• GO:0032508 DNA duplex unwinding
• GO:0033567 DNA replication, Okazaki fragment processing
• GO:0043137 DNA replication, removal of RNA primer
• GO:0043504 mitochondrial DNA repair
• GO:0045740 positive regulation of DNA replication
• GO:0090304 nucleic acid metabolic process
• GO:1902990 mitotic telomere maintenance via semi-conservative replication

Cellular Component

• GO:0000781 chromosome, telomeric region
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005739 mitochondrion
• GO:0005760 gamma DNA polymerase complex
• GO:0042645 mitochondrial nucleoid

External links

• PDB: RCSB:5ean, PDBe:5ean, PDBj:5ean
• PDBsum: 5ean
• PubMed: 26491943
• UniProt: Q6ZQJ5|DNA2_MOUSE DNA replication ATP-dependent helicase/nuclease DNA2 (Gene Name=Dna2)