Structure of PDB 5e63 Chain A

Receptor sequence

>5e63A (length=293) Species: 771870 (Sordaria macrospora k-hell)

LNPWAVVGFIDAEGSFMVRVRKNSKYKTGWLVVAIFSVTVDKKDLFLLES
LKTFFGGLGSIKKSGNSTFSYRIESSEQLTKIILPFFDKYSLITEKLGDY
LLFKKVLELMGTKEHLTQRGLEKIVSLKASINKGLSEELQAAFPQCVPTP
RPEINNKNIPDPFWLAGFVSGDGSFKSILKKSESIKVGFQSILVFQITQH
ARDVKLMESLISYLGCGFIEKDSRGPWLYYTVTNFSDIQGKIIPFFHQYK
IIGSAYGDYQDWCKIALIMQNKNHLTPEGLNEIRALKGGMNKG

3D structure

• PDB: 5e63 The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G178 D179
• Catalytic site (residue number reindexed from 1): G171 D172

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E20 V47 D48 S191 I192 K193 Q197 E227 R231 T240 N241 F242 H281 L282	E13 V40 D41 S184 I185 K186 Q190 E220 R224 T233 N234 F235 H274 L275
BS02	dna	A	A19 S22 M24 R26 R28 T46 R79 K103 N139 K140 D179	A12 S15 M17 R19 R21 T39 R72 K96 N132 K133 D172
BS03	dna	A	K32 Y33 K34 K69 S71 E81 S82 H122 L123 D179 T205 Q206 H207 W234	K25 Y26 K27 K62 S64 E74 S75 H115 L116 D172 T198 Q199 H200 W227
BS04	dna	A	E20 G178 S181 K187 K294	E13 G171 S174 K180 K287
BS05	MG	A	E20 D179	E13 D172
BS06	MG	A	A19 D179	A12 D172

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity

External links

• PDB: RCSB:5e63, PDBe:5e63, PDBj:5e63
• PDBsum: 5e63
• PubMed: 26705195
• UniProt: F7WD42