Structure of PDB 5e5s Chain A

Receptor sequence

>5e5sA (length=297) Species: 771870 (Sordaria macrospora k-hell)

ENSKLNPWAVVGFIDAEGSFMVRVRKNSKYKTGWLVVAIFSVTVDKKDLF
LLESLKTFFGGLGSIKKSGNSTFSYRIESSEQLTKIILPFFDKYSLITEA
LGDYLLFKKVLELMGTKEHLTQRGLEKIVSLKASINKGLSEELQAAFPQC
VPTPRPEINNKNIPDPFWLAGFVSGDGSFKSILKKSESIKVGFQSILVFQ
ITQHARDVKLMESLISYLGCGFIEKDSRGPWLYYTVTNFSDIQGKIIPFF
HQYKIIGSKYGDYQDWCKIALIMQNKNHLTPEGLNEIRALKGGMNKG

3D structure

• PDB: 5e5s The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.
• Chain: A
• Resolution: 2.29 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G178 D179
• Catalytic site (residue number reindexed from 1): G175 D176

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E20 M24 R26 R28 T46 D48 S74 T75 R79 N139 K187 S191 I192 K193 Q197 F225 R231 N241 F242 H281 L282	E17 M21 R23 R25 T43 D45 S71 T72 R76 N136 K184 S188 I189 K190 Q194 F222 R228 N238 F239 H278 L279
BS02	dna	A	K32 Y33 K34 K69 S71 E81 S82 S83 H122 D179 T205 Q206 H207 W234	K29 Y30 K31 K66 S68 E78 S79 S80 H119 D176 T202 Q203 H204 W231
BS03	dna	A	G178 S181 K183 I185 K187 K262 K294 N298	G175 S178 K180 I182 K184 K259 K291 N295
BS04	MG	A	A19 D179	A16 D176
BS05	MG	A	E20 G178	E17 G175

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity

External links

• PDB: RCSB:5e5s, PDBe:5e5s, PDBj:5e5s
• PDBsum: 5e5s
• PubMed: 26705195
• UniProt: F7WD42