Structure of PDB 5e5o Chain A

Receptor sequence

>5e5oA (length=294) Species: 771870 (Sordaria macrospora k-hell)

KLNPWAVVGFIDAEGSFMVRVRKNSKYKTGWLVVAIFSVTVDKKDLFLLE
SLKTFFGGLGSIKKSGNSTFSYRIESSEQLTKIILPFFDKYSLITEKLGD
YLLFKKVLELMGTKEHLTQRGLEKIVSLKASINKGLSEELQAAFPQCVPT
PRPEINNKNIPDPFWLAGFVSGDGSFKSILKKSESIKVGFQSILVFQITQ
HARDVKLMESLISYLGCGFIEKDSRGPWLYYTVTNFSDIQGKIIPFFHQY
KIIGSKYGDYMDWCKIALIMQNKNHLTPEGLNEIRALKGGMNKG

3D structure

• PDB: 5e5o The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.
• Chain: A
• Resolution: 2.36 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G178 D179
• Catalytic site (residue number reindexed from 1): G172 D173

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E20 M24 R26 R28 T46 V47 D48 R79 N139 S191 I192 K193 Q197 F225 R231 T240 N241 F242 H281 L282	E14 M18 R20 R22 T40 V41 D42 R73 N133 S185 I186 K187 Q191 F219 R225 T234 N235 F236 H275 L276	PDBbind-CN: Kd~150nM
BS02	dna	A	K32 Y33 K34 K69 S71 E81 S82 S83 H122 D179 S181 K183 L186 K187 T205 H207 W234 N298	K26 Y27 K28 K63 S65 E75 S76 S77 H116 D173 S175 K177 L180 K181 T199 H201 W228 N292	PDBbind-CN: Kd~150nM
BS03	CA	A	E20 G178 D179	E14 G172 D173
BS04	CA	A	A19 E20 D179	A13 E14 D173
BS05	CA	A	D106 I138 R209	D100 I132 R203

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity

External links

• PDB: RCSB:5e5o, PDBe:5e5o, PDBj:5e5o
• PDBsum: 5e5o
• PubMed: 26705195
• UniProt: F7WD42