Structure of PDB 5e3h Chain A

Receptor sequence

>5e3hA (length=642) Species: 9606 (Homo sapiens)

FKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQK
GKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVEN
NDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNY
LDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIA
TVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRIC
KDLEFGTQKYEQWIVTVQKACMVFDKDEESRICKALFLYTSHLRKYNDAL
IISEHARMKDALDYLKDFFSNVRAEIEQDLTQRFEEKLQELESVSRDPSN
ENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFL
KPGILTGRGTGMTLPAQKCILDAFKDHNILIATSVADEGIDIAQCNLVIL
YEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDS
ILRLQTWDEAVFREKILHIQTHEKFIRDSQEVPDKENKKLLCRKCKALAC
YTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARCS
HDWGIHVKYKTFEIPVIKIESFTLYSKWKDFHFEKIPFDPAE

3D structure

• PDB: 5e3h Structural basis of RNA recognition and activation by innate immune receptor RIG-I.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K379 Q380 H381 Q507 Q511 N720 H830 F853 I875 V886 K888 W908	K139 Q140 H141 Q258 Q262 N456 H562 F585 I605 V616 K618 W628	PDBbind-CN: Kd=0.02nM
BS02	rna	A	N298 I300 S325 G326 T347 Q349 E510 K635 T636 R637 G663 R664 T697 S698 V699 F853 S906	N58 I60 S85 G86 T107 Q109 E261 K379 T380 R381 G407 R408 T433 S434 V435 F585 S626	PDBbind-CN: Kd=0.02nM
BS03	ZN	A	C810 C864 C869	C542 C596 C599
BS04	ADP	A	F241 R244 Q247 G267 G269 K270 T271	F1 R4 Q7 G27 G29 K30 T31
BS05	BEF	A	T266 K270 E373 E702	T26 K30 E133 E438

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5e3h, PDBe:5e3h, PDBj:5e3h
• PDBsum: 5e3h
• PubMed: 21947008
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)