Structure of PDB 5d6j Chain A

Receptor sequence
>5d6jA (length=630) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence]
MPFHNPFIKDGQIKFPDGSSIVAHVERWAKVRGDKLAYRFLDFSTERDGV
PRDLTWAQFSARNRAVAARLQQVTQPGDRVAILCPQNLDYLVAFFGALYA
GRIAVPLFDPSEPGHVGRLHAVLDNCHPSAILTTTEAAEGVRKFFRTRPA
NQRPRVIAVDAVPDDVASTWVNPDEPDETTIAYLQYTSGSTRIPTGVQIT
HLNLATNVVQVIEALEGEEGDRGLSWLPFFHDMGLITALLAPMIGHYFTF
MTPAAFVRRPERWIRELARKEGDTGGTISVAPNFAFDHAAARGVPKPGSP
PLDLSNVKAVLNGSEPISAATVRRFNEAFGPFGFPPKAIKPSYGLAEATL
FVSTTPSAEEPKIITVDRDQLNSGRIVEVDADSPKAVAQASAGKVGIAEW
AVIVDAESATELPDGQVGEIWISGQNMGTGYWGKPEESVATFQNILKSRT
NPSHAEGATDDATWVRTGDYGAFYDGDLYITGRVKDLVIIDGRNHYPQDL
EYSAQEASKAIRTGYVAAFSVPANQLPDEVFENAHSGIKRDPDDTSEQLV
IVAERAPGAHKLDIGPITDDIRAAIAVRHGVTVRDVLLTAAGAIPRTSSG
KIGRRACRAAYLDGSLRAGKVANDFPDATD
3D structure
PDB5d6j Crystal structure of FadD32, an enzyme essential for mycolic acid biosynthesis in mycobacteria.
ChainA
Resolution2.25 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N207 H231 A346
Catalytic site (residue number reindexed from 1) N207 H231 A346
Enzyme Commision number 6.2.1.20: long-chain-fatty-acid--[acyl-carrier-protein] ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A T187 S188 G189 S190 T191 R192 S314 E315 P316 S342 G344 L345 A346 D469 I480 R483 T187 S188 G189 S190 T191 R192 S314 E315 P316 S342 G344 L345 A346 D469 I480 R483 MOAD: Kd=36uM
PDBbind-CN: -logKd/Ki=4.44,Kd=36uM
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008922 long-chain fatty acid [acyl-carrier-protein] ligase activity
GO:0016874 ligase activity
GO:0070566 adenylyltransferase activity
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006633 fatty acid biosynthetic process
GO:0008610 lipid biosynthetic process
Cellular Component
GO:0005886 plasma membrane

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Cellular Component
External links
PDB RCSB:5d6j, PDBe:5d6j, PDBj:5d6j
PDBsum5d6j
PubMed26628098
UniProtA0R618|FAA32_MYCS2 Long-chain-fatty-acid--AMP ligase FadD32 (Gene Name=fadD32)

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