Structure of PDB 5czz Chain A

Receptor sequence

>5czzA (length=1043)

RNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRG
ARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSE
EEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAE
LQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYI
DLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVKYAYN
ADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKE
ILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQIA
KILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAINL
ILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKR
SFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTN
ERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFN
YEVDHIIPRSVSFDNSFNNKVLVKQEEASKKGNRTPFQYLSSSDSKISYE
TFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFINRNLVDTRYA
TRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHHA
EDALIIANADFIFKEWKKLDKAKKVMENQMFMPEIETEQEYKEIFITPHQ
IKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLY
DKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYE
ETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSL
KPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKAYEEAKKLKKISNQ
AEFIASFYNNDLIKINGELYRVIGVNNDLLNRIEVNMIDITYREYLENMN
DKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIKK

3D structure

• PDB: 5czz Crystal Structure of Staphylococcus aureus Cas9.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	V41 N43 N44 R47 R48 K50 R51 R54 R55 L56 K57 R58 R59 R61 H62 R66 P88 Y89 H111 K114 R115 R116 G117 G162 E163 V164 R165 G166 N169 R170 R208 R209 T210 Y211 E213 G214 P215 G216 S219 F221 T238 Y239 K248 Y256 N257 R314 H393 N394 Q414 L446 R452 K459 R774 K778 N780 R781 E782 L783 L788 S790 R792 N804 L828 H832 D833 K867 Y868 S869 K870 P875 V876 K878 K879 I880 K881 Y897 S900 R901 V904 K906 K935	V39 N41 N42 R45 R46 K48 R49 R52 R53 L54 K55 R56 R57 R59 H60 R64 P86 Y87 H109 K112 R113 R114 G115 G160 E161 V162 R163 G164 N167 R168 R206 R207 T208 Y209 E211 G212 P213 G214 S217 F219 T236 Y237 K246 Y254 N255 R312 H391 N392 Q412 L444 R450 K457 R765 K769 N771 R772 E773 L774 L779 S781 R783 N795 L819 H823 D824 K858 Y859 S860 K861 P866 V867 K869 K870 I871 K872 Y888 S891 R892 V895 K897 K926
BS02	dna	A	N120 Y211 W229 Y230 L233 G235 R245 N264 G312 Y313 R314 V315 T390 G391 T392 N413 N419 L446 Y651 R654 N785 D786 T787 Y789 K815 Y882 R1012 P1013 T1019	N118 Y209 W227 Y228 L231 G233 R243 N262 G310 Y311 R312 V313 T388 G389 T390 N411 N417 L444 Y649 R652 N776 D777 T778 Y780 K806 Y873 R1003 P1004 T1010
BS03	dna	A	N885 K886 N888 A889 S908 L909 P911 I982 N985 R991 R1015	N876 K877 N879 A880 S899 L900 P902 I973 N976 R982 R1006

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:5czz, PDBe:5czz, PDBj:5czz
• PDBsum: 5czz
• PubMed: 26317473
• UniProt: J7RUA5|CAS9_STAAU CRISPR-associated endonuclease Cas9 (Gene Name=cas9)