Structure of PDB 5cwr Chain A

Receptor sequence

>5cwrA (length=325) Species: 9606 (Homo sapiens)

TNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEA
CSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTA
QMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTV
QKAAQAFNSGLLCVACGSYRRGKATCGDVDVAITHPDGRSHRGIFSRLLD
SLRQEGFLTDDLVSQEENGQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFA
CALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRV
LPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 5cwr Structural Mechanism for the Fidelity Modulation of DNA Polymerase lambda
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D178 D180 D240
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 R514 R517 K521 L527 S528 E529 H530	W25 V213 R264 R267 K271 L277 S278 E279 H280
BS02	dna	A	W342 G343 A344 G345 K347 T348 R488 D490 Y505	W93 G94 A95 G96 K98 T99 R238 D240 Y255
BS03	dna	A	G548 R549	G298 R299
BS04	dna	A	Y267 W274 R275 Y279 P303 G304 G306 R308 M309 K312	Y18 W25 R26 Y30 P54 G55 G57 R59 M60 K63
BS05	DCP	A	R386 G416 S417 R420 G426 D427 D429 Y505 F506 T507 G508 A510 N513	R137 G167 S168 R171 G177 D178 D180 Y255 F256 T257 G258 A260 N263
BS06	CA	A	D427 D429 D490	D178 D180 D240
BS07	CA	A	D427 D429	D178 D180

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:5cwr, PDBe:5cwr, PDBj:5cwr
• PDBsum: 5cwr
• PubMed: 26836966
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)