Structure of PDB 5cvu Chain A

Receptor sequence

>5cvuA (length=354) Species: 36903 (Clarkia breweri)

HSSDEEANLFAMQLASAAVLPMALKAAIELDVLEIMAKSVPPSGYISPAE
IAAQLPTTNPEAPVMLDRVLRLLASYSVVTYTLRELPSGKVERLYGLAPV
CKFLTKNEDGVSLAPFLLLATDKVLLEPWFYLKDAILEGGIPFNKAYGMN
IWDYFGTDHRINKVFNKGMSSNSTITMKKILEMYNGFEGLTTIVDVGGGT
GAVASMIVAKYPSINAINFDLPHVIQDAPAFSGVEHLGGDMFDGVPKGDA
IFIKWICHDWSDEHCLKLLKNCYAALPDHGKVIVAEYILPPSPDPSIATK
VVIHTDALMLAYNPGGKERTEKEFQALAMASGFRGFKVASCAFNTYVMEF
LKTA

3D structure

• PDB: 5cvu Structure of sinapyl alcohol bound monolignol 4-O-methyltransferase at 1.60 Angstroms resolution
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H272 D273 E300 E332
• Catalytic site (residue number reindexed from 1): H258 D259 E286 E318
• Enzyme Commision number: 2.1.1.146: (iso)eugenol O-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	55B	A	A134 W166 F179 W269 H272 D273 M323 Y326 N327	A120 W152 F165 W255 H258 D259 M309 Y312 N313
BS02	SAH	A	W166 F179 M183 S187 G211 D234 L235 D254 M255 K268 I270	W152 F165 M169 S173 G197 D220 L221 D240 M241 K254 I256

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0008171 O-methyltransferase activity
• GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
• GO:0046983 protein dimerization activity
• GO:0050630 (iso)eugenol O-methyltransferase activity

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:5cvu, PDBe:5cvu, PDBj:5cvu
• PDBsum: 5cvu
• UniProt: O04385|IEMT_CLABR (Iso)eugenol O-methyltransferase (Gene Name=IEMT1)