Structure of PDB 5ch7 Chain A

Receptor sequence

>5ch7A (length=894) Species: 640081 (Azospira oryzae PS)

SGAFEYSGWENFHRTQWSWDKKTRGAHLVNCTGACPHFVYSKDGVVMREE
QSKDIAPMPNIPEYNPRGCNKGECGHDYMYGPHRIKYPLIRVGERGEGKW
RRATWEEALDMIADKCVDTIKNHAPDCISVYSPVPAVSPVSFSAGHRFAH
YIGAHAHTFYDWYGDHPTGQTQTCGVQGDTCETADWFNSKYIILWGSNPT
QTRIPDAHFLSEAQLNGAKIVSISPDYNSSTIKVDKWIHPQPGTDGALAM
AMAHVIIKEKLYDAHSLKEQTDLSYLVRSDTKRFLREADVVAGGSKDKFY
FWNAKTGKPVIPKGSWGDQPEKKGSPVGFLGRNTFAFPKGYIDLGDLDPA
LEGKFNMQLLDGKTVEVRPVFEILKSRLMADNTPEKAAKITGVTAKAITE
LAREFATAKPSMIICGGGTQHWYYSDVLLRAMHLLTALTGTEGTNGGGMN
HYIGQWKPAFVAGLVALAFPEGVNKQRFCQTTIWTYIHAEVNDEIISSDI
DTEKYLRDSITTGQMPNMPEQGRDPKVFFVYRGNWLNQAKGQKYVLENLW
PKLELIVDINIRMDSTALYSDVVLPSAHWYEKLDLNVTSEHSYINMTEPA
IKPMWESKTDWQIFLALAKRVEMAAKRKKYEKFNDEKFKWVRDLSNLWNQ
MTMDGKLAEDEAAAQYILDNAPQSKGITIQMLREKPQRFKSNWTSPLKEG
VPYTPFQYFVVDKKPWPTLTGRQQFYLDHDTFFDMGVELPTYKAPIDADK
YPFRFNSPHSRHSVHSTFKDNVLMLRLQRGGPSIEMSPLDAKPLGIKDND
WVEAWNNHGKVICRVKIRNGEQRGRVSMWHCPELYMDLLTGGSQSVCPVR
INPTNLVGNYGHLFFRPNYYGPAGSQRDVRVNVKRYIGATPISF

3D structure

• PDB: 5ch7 Perchlorate Reductase Is Distinguished by Active Site Aromatic Gate Residues.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K76 P138 P140 W167 Y168 D170 P172 Y457 Q460
• Catalytic site (residue number reindexed from 1): K71 P133 P135 W162 Y163 D165 P167 Y452 Q455

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	H32 C36 G38 C40 G73 C74 G77	H27 C31 G33 C35 G68 C69 G72
BS02	MO	A	D170 G459	D165 G454
BS03	MGD	A	D170 G201 N203 Q206 T207 I228 P230 D231 N233 D250 G421 G422 G423 W427 I458 G459 N761 P763 H764 S765 S768 V769 H770 R830 R882	D165 G196 N198 Q201 T202 I223 P225 D226 N228 D245 G416 G417 G418 W422 I453 G454 N756 P758 H759 S760 S763 V764 H765 R825 R877
BS04	MD1	A	Y168 D170 H426 R537 G538 N539 Q543 I564 N565 I566 R567 D569 S581 W584 K587 D615 S762 H764 H770 S771 T772 H835 Q849 Q881	Y163 D165 H421 R532 G533 N534 Q538 I559 N560 I561 R562 D564 S576 W579 K582 D610 S757 H759 H765 S766 T767 H830 Q844 Q876
BS05	ZN	A	H81 D82	H76 D77
BS06	SO3	A	N804 P896 S898	N799 P891 S893

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009061 anaerobic respiration
• GO:0045333 cellular respiration

Cellular Component

• GO:0016020 membrane
• GO:1990204 oxidoreductase complex

External links

• PDB: RCSB:5ch7, PDBe:5ch7, PDBj:5ch7
• PDBsum: 5ch7
• PubMed: 26940877
• UniProt: G8QM55